Hiramatsu K, Miyatani S, Kim M, Yamada S, Okumura K, Tada T
J Immunol. 1981 Sep;127(3):1118-22.
Hybrid cell lines were established by fusion between keyhole limpet hemocyanin(KLH) binding T cells of A/J mice and an AKR T cell tumor line, BW5147. Hybrids were selected for the presence of Ia antigen and KLH-specific augmenting activity of their extracts in the secondary antibody response. The detailed phenotypic and functional analysis of 1 of these clones, FL10, is reported here. The hybrid was positive for both Thy1.1 and Thy1.2 antigens and possessed the Lyt-1+,2-,3- phenotype. Both VH and Ia determinants were detected on their cell surface. The IA locus was mapped in the I-A subregion, but the Ia specificities were serologically distinct from those of B cell Ia antigen. This was demonstrated by the fact that anti-Ia antiserum preabsorbed with B cells could react with the hybrid cells, whereas none of the monoclonal anti-Ia specific for private and public determinations of Iak could. The extract from the cell line specifically augmented the in vitro secondary antibody response against dinitrophenylated KLH, and this activity was removed by absorption with antigen and conventional anti-Ia antisera. The results indicate that the cell line, FL10, carries Ia antigen unique to the T cell, which is associated with the antigen-specific augmenting molecule.
通过将A/J小鼠的钥孔戚血蓝蛋白（KLH）结合性T细胞与AKR T细胞肿瘤系BW5147融合，建立了杂交细胞系。选择杂交体是因为其提取物在二次抗体应答中存在Ia抗原和KLH特异性增强活性。本文报道了其中一个克隆FL10的详细表型和功能分析。该杂交体Thy1.1和Thy1.2抗原均呈阳性，具有Lyt-1 +、2 -、3 -表型。在其细胞表面检测到VH和Ia决定簇。IA基因座定位于I-A亚区，但Ia特异性在血清学上与B细胞Ia抗原不同。这一点通过以下事实得以证明：用B细胞预先吸收的抗Ia抗血清可与杂交细胞反应，而针对Iak的私有和公有决定簇的单克隆抗Ia抗体均不能。该细胞系的提取物特异性增强了针对二硝基苯基化KLH的体外二次抗体应答，并且这种活性通过用抗原和传统抗Ia抗血清吸收而去除。结果表明，细胞系FL10携带T细胞特有的Ia抗原，该抗原与抗原特异性增强分子相关。
