Duncan M E
Cytobios. 1977;19(73):45-66.
Mutants of human lymphoblastoid cell lines have been isoalted, which are resistant to 6-thioguanine or to 2,6-diaminopurine. They have been partially characterized and shown to be almost totally deficient in HGPRT and APRT, respectively. Cell lines doubly mutant for HGPRT deficiency and resistance to ouabain have also been isolated. All mutants were indistinguishable from the respective parental lines by criteria other than those leading specifically to their drug resistant phenotypes. The use of these mutants in the production of hybrids between two lines of suspension-growing human lymphoblastoid cells is discussed.
已分离出对6-硫鸟嘌呤或2,6-二氨基嘌呤具有抗性的人淋巴母细胞系突变体。它们已得到部分表征，分别显示出几乎完全缺乏次黄嘌呤-鸟嘌呤磷酸核糖转移酶（HGPRT）和腺嘌呤磷酸核糖转移酶（APRT）。还分离出了HGPRT缺陷和对哇巴因抗性的双突变细胞系。除了那些导致其耐药表型的标准外，所有突变体与各自的亲代细胞系在其他标准上无法区分。讨论了利用这些突变体在两株悬浮生长的人淋巴母细胞系之间产生杂种的情况。
