Goyanes V J, Mendez J
Cancer Genet Cytogenet. 1981 Aug;4(1):45-51. doi: 10.1016/0165-4608(81)90007-8.
A BrdU-Hoechst 33258 treatment of living cells, which selectively induced condensation-inhibition of G-band chromatin in human and Chinese hamster chromosomes, is presented. As a consequence mitotic chromosomes showed high resolution R-banding patterns when examined by light and electron microscopy. Besides each whole chromosome identification, this procedure also permitted the electron microscopic study of specific structures, such as satellites, secondary constrictions, telomeres, centromeres, as well as G and R bands, some of them no visible by light microscopy. We have also observed that the chromatin of G and R bands behave as blocks of chromatin condensation and that G-band chromatin develops condensation along G2. Under the BrdU-Hoechst 33258 treatment, chromatin fibers seem to invert their spontaneous pattern of condensation within the chromosomes.
本文介绍了一种用溴脱氧尿苷（BrdU）- Hoechst 33258处理活细胞的方法，该方法能选择性地诱导人类和中国仓鼠染色体中G带染色质的凝聚抑制。结果，当通过光学显微镜和电子显微镜检查时，有丝分裂染色体呈现出高分辨率的R带模式。除了能对每条完整染色体进行识别外，该方法还允许对特定结构进行电子显微镜研究，如随体、次缢痕、端粒、着丝粒以及G带和R带，其中一些在光学显微镜下不可见。我们还观察到，G带和R带的染色质表现为染色质凝聚块，并且G带染色质在G2期沿其发展凝聚。在BrdU - Hoechst 33258处理下，染色质纤维似乎在染色体内反转了它们自发的凝聚模式。
