Kato T, Minagawa T
Microbiol Immunol. 1981;25(8):837-45. doi: 10.1111/j.1348-0421.1981.tb00087.x.
Human lung carcinoma cells persistently infected with mumps virus (Pc-10/MpV) were lysed with human peripheral blood mononuclear leukocytes (PBML) obtained from seropositive donors who had anti-mumps virus-neutralizing antibody in their sera. This cellular cytotoxicity was due not to the cytotoxic T lymphocytes but mainly to the non-T, non-B cells, possibly related to natural killer (NK) cells. Moreover, it was concerned not with antibody against mumps virus antigens but with alpha-interferon (IFN-alpha) produced in the mixture of human PBML and Pc-10/MpV cells, since this cellular cytotoxicity was suppressed by anti-human IFN-alpha rabbit serum. Exogeneous IFN-alpha augmented the cytotoxicity of non-T, non-B cells, not T cells, for the uninfected Pc-10 cells. IFN-gamma that had been induced by heat-killed Listeria monocytogenes in PBML had the same capacity to augment NK activity did IFN-alpha.
用人外周血单个核白细胞（PBML）裂解持续感染腮腺炎病毒的人肺癌细胞（Pc-10/MpV），PBML取自血清阳性供体，这些供体血清中含有抗腮腺炎病毒中和抗体。这种细胞毒性不是由细胞毒性T淋巴细胞引起的，而是主要由非T、非B细胞引起的，可能与自然杀伤（NK）细胞有关。此外，它与抗腮腺炎病毒抗原的抗体无关，而是与人PBML和Pc-10/MpV细胞混合物中产生的α-干扰素（IFN-α）有关，因为这种细胞毒性被抗人IFN-α兔血清所抑制。外源性IFN-α增强了非T、非B细胞而非T细胞对未感染的Pc-10细胞的细胞毒性。PBML中经热灭活的单核细胞增生李斯特菌诱导产生的IFN-γ与IFN-α具有相同的增强NK活性的能力。
