Overbeek P A, Merlino G T, Peters N K, Cohn V H, Moore G P, Kleinsmith L J
Biochim Biophys Acta. 1981 Dec 28;656(2):195-205. doi: 10.1016/0005-2787(81)90087-3.
Hybridization of an actin cDNA clone (pSA38) to restriction enzyme digests of Strongylocentrotus purpuratus DNA indicates that the sea urchin genome contains at least five different actin genes. A sea urchin genomic clone library was screened for recombinants which hydridize to pSA38 and four genomic clones were isolated. Restriction maps were generated which indicate that three of these recombinants contain different actin genes, and that the fourth may be an allele to one of these. The restriction maps suggest that one clone contains two linked actin genes. This fact, which was confirmed by heteroduplex analysis, indicates that the actin gene family may be clustered. The linked genes are oriented in the same direction and spaced about 8.0 kilobases apart. In heteroduplexes between genomic clones two intervening sequences were seen. Significant homology is confined to the actin coding region and does not include any flanking sequence. Southern blot analysis reveals that repetitive DNA sequences are found in the region of the actin genes.
用肌动蛋白cDNA克隆（pSA38）与紫球海胆（Strongylocentrotus purpuratus）DNA的限制性酶切片段杂交，结果表明海胆基因组至少含有5种不同的肌动蛋白基因。对海胆基因组克隆文库进行筛选，寻找能与pSA38杂交的重组体，分离出了4个基因组克隆。构建了限制性图谱，结果显示其中3个重组体含有不同的肌动蛋白基因，第4个可能是其中一个的等位基因。限制性图谱表明，一个克隆含有两个连锁的肌动蛋白基因。这一事实经异源双链分析得以证实，表明肌动蛋白基因家族可能成簇排列。连锁基因的方向相同，间隔约8.0千碱基对。在基因组克隆之间的异源双链体中可见两个间隔序列。显著的同源性仅限于肌动蛋白编码区，不包括任何侧翼序列。Southern印迹分析显示，在肌动蛋白基因区域发现了重复DNA序列。
