Effects of the positively regulating product of gene 28 of the B. subtilis phage SPO1 on in vitro transcription.

Some of the properties of the RNA polymerase purified from SPO1-infected Bacillus subtilis have been compared with the properties of RNA polymerase from uninfected cells (core + sigma). The two enzymes synthetize RNA from nonoverlapping regions on SPO1 DNA, and they lead to the retention of different restriction fragments of SPO1 DNA on cellulose-nitrate filters. The action of the positively regulating product of gene 28 of SPO1 (gp 28) has been analyzed. The isolated gp 28 has been shown to be unable to increase the dissociation rate of core + sigma from SPP1 DNA, while it efficiently blocks the initiation of RNA synthesis if it is added to performed complexes between core + sigma and SPP1 DNA in 0.2 M NaCl.