Uyemura K, Suzuki M, Kitamura K, Horie K, Ogawa Y, Matsuyama H, Nozaki S, Muramatsu I
J Neurochem. 1982 Sep;39(3):895-8. doi: 10.1111/j.1471-4159.1982.tb07979.x.
Experimental allergic neuritis (EAN) is an experimentally produced demyelinating disease of peripheral nervous system. Several peptides of bovine P2 protein were tested for neuritogenic activity in Lewis rats. The hexacosapeptide CiT4 (residues 53--78 of bovine P2 protein) showed the highest neuritogenic activity among the peptides tested. The nonapeptide (residues 70--78) and the tridecapeptide (residues 66--78) were synthesized using the liquid phase peptide synthesis technique. The tridecapeptide showed mild, but definite activity in inducing EAN in the rats, while the nonapeptide was inactive. The localization of the neuritogenic determinant of bovine P2 protein in Lewis rats is discussed.
实验性变应性神经炎（EAN）是一种通过实验诱导产生的周围神经系统脱髓鞘疾病。对牛P2蛋白的几种肽段进行了检测，以评估其在Lewis大鼠中的致神经炎活性。在测试的肽段中，二十六肽CiT4（牛P2蛋白的53-78位氨基酸残基）显示出最高的致神经炎活性。使用液相肽合成技术合成了九肽（70-78位氨基酸残基）和十三肽（66-78位氨基酸残基）。十三肽在诱导大鼠发生EAN方面表现出轻微但确切的活性，而九肽则无活性。本文讨论了牛P2蛋白致神经炎决定簇在Lewis大鼠中的定位。
