Ruben L, Hooper A B
Mol Cell Biol. 1982 May;2(5):508-16. doi: 10.1128/mcb.2.5.508-516.1982.
The antibiotic chloramphenicol selectively inhibited mitochondrial protein synthesis in the ciliate protozoan Tetrahymena pyriformis GL. Secondary to the inhibition of mitochondrial protein synthesis was an inhibition of nuclear RNA synthesis at a time before inhibition of cellular protein and DNA synthesis. Of the stable non-polyadenylated RNA species in Tetrahymena, the addition of chloramphenicol resulted specifically in the inhibition of synthesis of 28S + 17S and 5S rRNA transcripts. By contrast, syntheses of 4S tRNA and 21S mitochondrial rRNA were not as extensively inhibited. The addition of 60 microM hemin before the addition of chloramphenicol partially protected against the inhibition of RNA synthesis. These data indicate that continued synthesis of nucleus-directed rRNA is linked to the synthesis of mitochondrial proteins in Tetrahymena.
抗生素氯霉素选择性地抑制了梨形四膜虫GL中的线粒体蛋白质合成。在线粒体蛋白质合成受到抑制之后，在细胞蛋白质和DNA合成受到抑制之前的某个时间点，核RNA合成也受到了抑制。在四膜虫中稳定的非聚腺苷酸化RNA种类中，添加氯霉素特别导致28S + 17S和5S rRNA转录本的合成受到抑制。相比之下，4S tRNA和21S线粒体rRNA的合成没有受到如此广泛的抑制。在添加氯霉素之前添加60 microM血红素可部分防止RNA合成受到抑制。这些数据表明，在四膜虫中，由细胞核指导的rRNA的持续合成与线粒体蛋白质的合成相关联。
