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[受体的分子机制。II. 视紫红质构象转变的鉴定,该转变与由视杆细胞外段片段修饰的人工脂质膜光反应前沿有关]

[Molecular mechanisms of receptor. II. Identification of the conformational transition of rhodopsin responsible for the leading edge of the photoresponse of artificial lipid membranes modified by fragments of the outer segment of rods].

作者信息

Fesenko E E, Orlov N Ia, Ratner V L, Liubarskiĭ A L

出版信息

Mol Biol (Mosk). 1977 Jul-Aug;11(4):741-7.

PMID:618319
Abstract

Kinetic parameters of photoinduced permeability increase of artificial lipid membranes, modified by ROS fragments (tau20 degrees C = 20 mesec Ea = 33 +/- 2 kcal/mole) coincides with appropriate parameters of photoinduced protein fluorescence intensity decrease and ROS fragments absorption spectra change (metarhodopsin I leads leads to metarhodopsin II transition). Hydroxylamine accelerates this process, its rate is proportional to hydroxylamine at concentrations lower than 0.6 M.

摘要

由活性氧片段修饰的人工脂质膜光诱导通透性增加的动力学参数(20℃时τ = 20毫秒,Ea = 33±2千卡/摩尔)与光诱导蛋白质荧光强度降低和活性氧片段吸收光谱变化(视紫红质I向视紫红质II转变)的相应参数一致。羟胺加速了这一过程,在浓度低于0.6 M时其速率与羟胺成正比。

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