Marcel Y L, Hogue M, Theolis R, Milne R W
J Biol Chem. 1982 Nov 25;257(22):13165-8.
The reactivity of a series of monoclonal antibodies directed against human low density lipoproteins (LDL) has been tested with hepatic and intestinal apolipoprotein B (apo-B) termed B-100 and B-48, respectively (Kane, J. P., Hardman, D. A., and Paulus, H. E. (1980) Proc. Natl. Acad. Sci. U. S. A. 77, 2465-2469). Whereas those antibodies that have been previously shown to recognize determinants close to the LDL receptor recognition site reacted only with B-100, two antibodies specific for other regions of apo-B reacted with both B-100 and B-48. Therefore, it is probable that sequence homologies exist between the two proteins and it must be considered that all or parts of the B-48 sequence may be contained within that of B-100. The specificity of the reaction of these antibodies with proteins designated B-74 and B-26 supports the concept that they represent complementary fragments of B-100. The present results have been incorporated in a theoretical map of the antigenic determinants recognized by these antibodies on the LDL apo-B.
一系列针对人低密度脂蛋白（LDL）的单克隆抗体，已分别与肝脏和肠道载脂蛋白B（apo - B）（分别称为B - 100和B - 48）进行了反应性测试（凯恩，J.P.，哈德曼，D.A.，和保卢斯，H.E.（1980年）《美国国家科学院院刊》77，2465 - 2469）。先前已证明能识别靠近LDL受体识别位点决定簇的那些抗体，仅与B - 100反应，而两种对apo - B其他区域特异的抗体，与B - 100和B - 48都反应。因此，这两种蛋白质之间可能存在序列同源性，并且必须考虑B - 48序列的全部或部分可能包含在B - 100序列之中。这些抗体与命名为B - 74和B - 26的蛋白质反应的特异性，支持了它们代表B - 100互补片段的概念。目前的结果已纳入这些抗体在LDL apo - B上识别的抗原决定簇的理论图谱中。
