Grond J, Schilthuis M S, Koudstaal J, Elema J D
Kidney Int. 1982 Oct;22(4):338-43. doi: 10.1038/ki.1982.178.
To investigate the possible relationship between disturbance of mesangial function and segmental localization of glomerular sclerosis, five uninephrectomized male Wistar rats and five sham-operated controls received colloidal carbon intravenously. At 4 months 8.4 +/- 2.5% of the glomeruli of the nephrectomized rats showed focal sclerosis. Glomeruli of nephrectomized rats contained significantly more carbon than glomeruli of controls. Glomeruli with focal sclerosis contained significantly more carbon than normal glomeruli in the same kidneys with a preferential tracer localization within the lesions. In another experiment carbon injections were given before surgery. At 4 months 12.6 +/- 4.1% of the glomeruli of the nephrectomized rats showed focal sclerosis, an incidence not significantly different from that of the first experiment. Glomerular carbon content was equal in experimental and control rats and no preferential localization of the tracer within the lesions was found. From these results we conclude that the preferential localization of carbon in the glomerular lesions in rats nephrectomized before injection of carbon is caused by the increased delivery of tracer shortly after injection to those glomerular areas where sclerosis will develop at a later time. The development of focal sclerosis may be related to the local deposition of harmful substances from the circulation.
为了研究肾小球系膜功能紊乱与肾小球硬化节段定位之间的可能关系，对5只单侧肾切除的雄性Wistar大鼠和5只假手术对照组大鼠静脉注射胶体碳。4个月时，肾切除大鼠的肾小球中有8.4±2.5%出现局灶性硬化。肾切除大鼠的肾小球含碳量明显高于对照组的肾小球。有局灶性硬化的肾小球比同一肾脏中的正常肾小球含碳量明显更多，且示踪剂优先定位于病变部位。在另一项实验中，在手术前注射碳。4个月时，肾切除大鼠的肾小球中有12.6±4.1%出现局灶性硬化，其发生率与第一个实验无显著差异。实验大鼠和对照大鼠的肾小球碳含量相等，且未发现示踪剂在病变部位有优先定位。从这些结果我们得出结论，在注射碳之前进行肾切除的大鼠中，碳在肾小球病变中的优先定位是由于注射后不久示踪剂向那些随后会发生硬化的肾小球区域的输送增加所致。局灶性硬化的发生可能与循环中有害物质的局部沉积有关。
