地塞米松对HeLa细胞中多萜醇介导的糖基化作用的刺激研究。
Studies on the stimulation of dolichol-mediated glycosylation by dexamethasone in HeLa cells.
作者信息
Ramachandran C K, Gray S L, Melnykovych G
出版信息
Biochem J. 1982 Oct 15;208(1):47-52. doi: 10.1042/bj2080047.
Abstract
The effect of the addition of 1 microM-dexamethasone on the temporal sequence of the glycosylation process has been studied in HeLa S3G cells. In the presence of delipidized serum, dexamethasone caused an increase in 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase and an accelerated synthesis of dolichols. These events were followed by an increase in the transfer of mannose from GDP-mannose to mannolipid. An increase in the rate of synthesis of lipid-linked oligosaccharides and a stimulation of glycosylation were observed in cells grown in the presence of delipidized serum in the culture medium. The data are consistent with the view that cellular syntheses of lipids and glycoproteins are co-ordinately controlled.
摘要
在HeLa S3G细胞中研究了添加1微摩尔地塞米松对糖基化过程时间顺序的影响。在脱脂血清存在的情况下，地塞米松导致3-羟基-3-甲基戊二酰辅酶A（HMG-CoA）还原酶增加，并加速了多萜醇的合成。这些事件之后是甘露糖从GDP-甘露糖向甘露糖脂的转移增加。在含有脱脂血清的培养基中生长的细胞中，观察到脂质连接寡糖合成速率增加和糖基化受到刺激。这些数据与脂质和糖蛋白的细胞合成受到协调控制的观点一致。
相似文献
1
Studies on the stimulation of dolichol-mediated glycosylation by dexamethasone in HeLa cells.地塞米松对HeLa细胞中多萜醇介导的糖基化作用的刺激研究。
Biochem J. 1982 Oct 15;208(1):47-52. doi: 10.1042/bj2080047.
2
Evidence for the stimulation of dolichol and mannolipid synthesis by glucocorticoids in HeLa cells.糖皮质激素刺激HeLa细胞中多萜醇和甘露糖脂合成的证据。
Biochem J. 1981 Jul 15;198(1):23-8. doi: 10.1042/bj1980023.
3
Inflammation-induced increases in dolichol synthesis and hydroxymethylglutaryl-coenzyme A reductase activity in mouse liver are prevented by a high-cholesterol diet but not by fasting.炎症诱导的小鼠肝脏中多萜醇合成增加及羟甲基戊二酰辅酶A还原酶活性增加可被高胆固醇饮食阻止，但不能被禁食阻止。
Biochim Biophys Acta. 1984 Dec 6;796(3):393-6. doi: 10.1016/0005-2760(84)90142-5.
4
Differential regulation of the synthesis of mannosylphosphoryl derivatives of dolichol and retinol in HeLa cells.HeLa细胞中多萜醇和视黄醇甘露糖基磷酸化衍生物合成的差异调节。
Biochim Biophys Acta. 1986 Jun 11;877(1):96-103. doi: 10.1016/0005-2760(86)90123-2.
5
Regulation of cholesterol biosynthesis in HeLa S3G cells by serum lipoproteins: dexamethasone-mediated interference with suppression of 3-hydroxy-3-methylglutaryl coenzyme A reductase.血清脂蛋白对HeLa S3G细胞胆固醇生物合成的调节：地塞米松介导的对3-羟基-3-甲基戊二酰辅酶A还原酶抑制作用的干扰。
Proc Natl Acad Sci U S A. 1978 May;75(5):2103-7. doi: 10.1073/pnas.75.5.2103.
6
Regulation of hepatic dolichol synthesis by beta-hydroxy-beta-methylglutaryl coenzyme A reductase.β-羟基-β-甲基戊二酰辅酶A还原酶对肝脏多萜醇合成的调节作用。
J Biol Chem. 1980 Sep 25;255(18):8618-22.
7
Role of biosynthesis of cholesterol and isoprenoid derivatives in regulation of G1 progression and cell proliferation of 3T6 cells.
J Cell Physiol. 1987 Oct;133(1):163-8. doi: 10.1002/jcp.1041330121.
8
Effect of dexamethasone on mannolipid synthesis by hepatocytes prepared from control and inflamed rats.地塞米松对从对照大鼠和炎症大鼠制备的肝细胞甘露脂合成的影响。
Biochem J. 1984 Apr 15;219(2):429-36. doi: 10.1042/bj2190429.
9
Effect of virus-transformation and growth factor stimulation on isoprene biosynthesis in human fibroblasts: a correlation to cell growth.
Cancer Biochem Biophys. 1995 Jan;14(4):243-56.
10
Abnormal synthesis of mannose 1-phosphate derived carbohydrates in carbohydrate-deficient glycoprotein syndrome type I fibroblasts with phosphomannomutase deficiency.在磷酸甘露糖变位酶缺乏的I型碳水化合物缺乏糖蛋白综合征成纤维细胞中，磷酸甘露糖衍生碳水化合物的异常合成。
Glycobiology. 1998 Feb;8(2):165-71. doi: 10.1093/glycob/8.2.165.
引用本文的文献
1
The role of substrate supply in the regulation of cholesterol biosynthesis in rat hepatocytes.底物供应在大鼠肝细胞胆固醇生物合成调节中的作用。
Biochem J. 1983 Mar 15;210(3):625-32. doi: 10.1042/bj2100625.
2
Effect of dexamethasone on mannolipid synthesis by hepatocytes prepared from control and inflamed rats.地塞米松对从对照大鼠和炎症大鼠制备的肝细胞甘露脂合成的影响。
Biochem J. 1984 Apr 15;219(2):429-36. doi: 10.1042/bj2190429.
3
Effects of bleomycin and methylprednisolone on the biosynthesis of oligosaccharide-lipids and glycoproteins in lung.博来霉素和甲基强的松龙对肺中寡糖脂和糖蛋白生物合成的影响。
Biochem J. 1983 Sep 1;213(3):609-16. doi: 10.1042/bj2130609.
本文引用的文献
1
Lipid intermediates in glycoprotein synthesis during rat liver regeneration.大鼠肝脏再生过程中糖蛋白合成的脂质中间体。
Int J Biochem. 1980;12(3):489-92. doi: 10.1016/0020-711x(80)90134-2.
2
Regulation of hepatic dolichol synthesis by beta-hydroxy-beta-methylglutaryl coenzyme A reductase.β-羟基-β-甲基戊二酰辅酶A还原酶对肝脏多萜醇合成的调节作用。
J Biol Chem. 1980 Sep 25;255(18):8618-22.
3
Modulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase by dexamethasone not mediated by phosphorylation and dephosphorylation.
Arch Biochem Biophys. 1980 Aug;203(1):117-22. doi: 10.1016/0003-9861(80)90159-9.
4
Evidence for the stimulation of dolichol and mannolipid synthesis by glucocorticoids in HeLa cells.糖皮质激素刺激HeLa细胞中多萜醇和甘露糖脂合成的证据。
Biochem J. 1981 Jul 15;198(1):23-8. doi: 10.1042/bj1980023.
5
Regulation of dolichol and of cholesterol biosynthesis in cholesterol-fed rabbits.
Arch Biochem Biophys. 1981 Apr 15;208(1):30-6. doi: 10.1016/0003-9861(81)90119-3.
6
Increased hepatic dolichol and dolichyl phosphate-mediated glycosylation in rats fed cholesterol.
Arch Biochem Biophys. 1981 Apr 1;207(2):427-36. doi: 10.1016/0003-9861(81)90050-3.
7
Changes in sterol biosynthesis accompanying cessation of glial cell growth in serum-free medium.无血清培养基中神经胶质细胞生长停止时甾醇生物合成的变化。
Biochem J. 1980 Nov 15;192(2):709-17. doi: 10.1042/bj1920709.
8
Cell-density-dependent changes in cell-surface glycopeptides and in adhesion of cultured intestinal epithelial cells.培养的肠上皮细胞的细胞表面糖肽及黏附中的细胞密度依赖性变化。
Biochem J. 1982 Feb 1;201(2):359-66. doi: 10.1042/bj2010359.
9
Transmembrane assembly of membrane and secretory glycoproteins.膜糖蛋白和分泌型糖蛋白的跨膜组装
Arch Biochem Biophys. 1981 Oct 1;211(1):1-19. doi: 10.1016/0003-9861(81)90423-9.
10
The activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase and the rate of sterol synthesis diminish in cultures with high cell density.在高细胞密度培养物中，3-羟基-3-甲基戊二酰辅酶A还原酶的活性和固醇合成速率会降低。
J Cell Physiol. 1981 Jul;108(1):91-7. doi: 10.1002/jcp.1041080112.
Zotero 插件