Rautela G S, Liedtke R J
Clin Chem. 1978 Jan;24(1):108-14.
We describe a completely automated enzymic system for measuring total cholesterol in serum. All reagents are contained in an analytical test pack and the test is performed on Du Pont's Automatic Clinical Analyzer (aca), which mixes the sample (20 microliter) and reagents and performs the necessary absorbance measurements and calculations. In the procedure, cholesterol oxidase oxidizes free cholesterol. The oxidation step produces cholest-4-en-3-one and hydrochloride peroxidesterase hydrolyzes cholesterol esters and cholesterol in direct proportion to the amount of cholesterol present. N,N-Diethylaniline hydrochloride and 4-aminoantipyrine react with the hydrogen peroxide to produce a quinoneimine dye (lambda max = 553 nm). Interacting reagents have been optimized simultaneously (coöptimization) utilizing response surface designs coupled with computer analysis of the data. Reagent efficiency is high and analytical performance reliable.
我们描述了一种用于测量血清中总胆固醇的全自动酶系统。所有试剂都包含在一个分析测试包中，该测试在杜邦自动临床分析仪（aca）上进行，该分析仪将样品（20微升）和试剂混合，并进行必要的吸光度测量和计算。在该过程中，胆固醇氧化酶氧化游离胆固醇。氧化步骤产生胆甾-4-烯-3-酮，盐酸过氧化物酶将胆固醇酯和胆固醇水解，水解量与存在的胆固醇量成正比。N，N-二乙基苯胺盐酸盐和4-氨基安替比林与过氧化氢反应生成醌亚胺染料（最大吸收波长=553nm）。利用响应面设计结合数据的计算机分析，对相互作用的试剂进行了同时优化（协同优化）。试剂效率高，分析性能可靠。
