Schmitz H E, Atassi H, Atassi M Z
Mol Immunol. 1982 Dec;19(12):1699-1702. doi: 10.1016/0161-5890(82)90282-6.
Two monoclonal antibodies with pre-selected submolecular binding specificities to sperm whale myoglobin (Mb) were obtained by hybridizing Fa/0 mouse myeloma cells with spleen cells derived from mice which had been immunized with free (not coupled to any carrier) Mb synthetic peptides 132-153 or 145-151 (antigenic site 5). Both monoclonal antibodies were IgG1 (k). Their homogeneity was confirmed by analytical isoelectric focusing electrophoresis. According to competitive inhibition studies in which Mb, the five synthetic antigenic sites of Mb, Mb peptide 132-153, bovine serum albumin (BSA), and lysozyme were used as inhibitors, the binding specificities of both monoclonal antibodies were restricted to determinants present in the peptides used for immunizations. The results of direct binding studies confirmed this conclusion and suggested that monoclonal antibodies with pre-selected submolecular binding specificities can be readily obtained by the techniques of somatic cell hybridization when the corresponding free synthetic determinants are used as immunogens.
通过将 Fa/0 小鼠骨髓瘤细胞与来自用游离（未偶联任何载体）的肌红蛋白（Mb）合成肽 132 - 153 或 145 - 151（抗原位点 5）免疫的小鼠的脾细胞杂交，获得了两种对抹香鲸肌红蛋白（Mb）具有预先选定的亚分子结合特异性的单克隆抗体。两种单克隆抗体均为 IgG1（κ）。通过分析等电聚焦电泳证实了它们的同质性。在竞争抑制研究中，使用 Mb、Mb 的五个合成抗原位点、Mb 肽 132 - 153、牛血清白蛋白（BSA）和溶菌酶作为抑制剂，结果表明两种单克隆抗体的结合特异性均局限于用于免疫的肽中存在的决定簇。直接结合研究的结果证实了这一结论，并表明当使用相应的游离合成决定簇作为免疫原时，通过体细胞杂交技术可以很容易地获得具有预先选定的亚分子结合特异性的单克隆抗体。
