Bendayan M, Zollinger M
J Histochem Cytochem. 1983 Jan;31(1):101-9. doi: 10.1177/31.1.6187796.
The protein A-gold immunocytochemical technique has been modified to allow labeling of cellular antigenic sites on osmium-fixed or postfixed tissues. Several strong oxidizing agents have been found able to restore protein antigenicity on osmicated tissue thin sections. According to the fine structural preservation and intensities of labeling, pretreatment with sodium metaperiodate gave optimal results. Pancreatic secretory proteins (and/or proproteins) as well as insulin (and/or proinsulin) were localized over perfectly preserved rough endoplasmic reticulum (rER), Golgi apparatus, and secretory granules of the corresponding pancreatic cells; carbamyl phosphate synthetase and catalase were revealed over liver mitochondria and peroxisomes, respectively. In addition to the higher resolution in the labeling obtained using osmium-fixed tissues, the present modification confers an additional advantage to the protein A-gold technique by allowing labeling on tissues processed for routine electron microscopy.
蛋白A-金免疫细胞化学技术已被改进，以允许对锇固定或后固定组织上的细胞抗原位点进行标记。已发现几种强氧化剂能够恢复经锇处理的组织薄片上的蛋白质抗原性。根据精细结构的保存情况和标记强度，用偏高碘酸钠预处理可获得最佳结果。胰腺分泌蛋白（和/或前体蛋白）以及胰岛素（和/或胰岛素原）定位于相应胰腺细胞保存完好的粗面内质网（rER）、高尔基体和分泌颗粒上；氨基甲酰磷酸合成酶和过氧化氢酶分别在肝线粒体和过氧化物酶体上显示出来。除了使用锇固定组织获得的标记具有更高分辨率外，本改进还赋予了蛋白A-金技术一个额外优势，即允许在用于常规电子显微镜检查的组织上进行标记。
