Siliciano R F, Brookmeyer R, Shin H S
J Immunol. 1983 Apr;130(4):1512-20.
Cytolytic and helper T cells exhibit, in addition to their specificity for foreign antigen, a restriction specificity for self MHC gene products. The present study was designed to assess the degree of diversity within the repertoire of receptors that are involved in T cell recognition of self MHC gene products. For this purpose, we generated a series of murine cytolytic T lymphocyte (CTL) clones specific for a hapten antigen and restricted to the self MHC gene product H-2Kb. An analysis of the hapten fine specificity of these clones by using hapten analogues revealed the presence of substantial diversity within the repertoire of CTL receptors specific for the hapten. The degree of diversity within the repertoire of self H-2 recognition structures on these clones was assessed by testing clones on panels of syngeneic, congenic H-2K disparate, and H-2Kb mutant target cells bearing varying amounts of antigen. A striking degree of heterogeneity in H-2K recognition was found among these H-2Kb restricted CTL. We estimate that there are probably a minimum of 65 different patterns of H-2K recognition among these clones. Our results suggest a high degree of diversity exists within the repertoire of self MHC recognition structures on antigen-specific T cells restricted to a single self MHC gene product.
除了对异体抗原具有特异性外，溶细胞性T细胞和辅助性T细胞对自身MHC基因产物还表现出限制性特异性。本研究旨在评估参与T细胞识别自身MHC基因产物的受体库中的多样性程度。为此，我们生成了一系列针对半抗原抗原且受自身MHC基因产物H-2Kb限制的小鼠溶细胞性T淋巴细胞（CTL）克隆。通过使用半抗原类似物对这些克隆的半抗原精细特异性进行分析，发现在针对半抗原的CTL受体库中存在大量多样性。通过在同基因、同源H-2K不同以及携带不同量抗原的H-2Kb突变靶细胞板上测试克隆，评估了这些克隆上自身H-2识别结构库中的多样性程度。在这些受H-2Kb限制的CTL中发现了H-2K识别方面显著程度的异质性。我们估计这些克隆中可能至少存在65种不同的H-2K识别模式。我们的结果表明，在受单一自身MHC基因产物限制的抗原特异性T细胞上的自身MHC识别结构库中存在高度多样性。
