Olianas M C, Onali P, Neff N H, Costa E
Mol Pharmacol. 1983 Mar;23(2):393-8.
Acetylcholine inhibits, by 30-40%, the basal adenylate cyclase activity of purified synaptic plasma membranes prepared from rat striatum (EC50 = 3 microM). Cholinergic receptor agonists inhibit this cyclase activity with the following rank order of potency: oxtremorine greater than acetylcholine greater than arecoline greater than methacholine greater than or equal to muscarine greater than or equal to carbachol greater than bethanechol. Nicotine fails to inhibit the cyclase, and d-tubocurarine fails to inhibit the action of cholinergic drugs. In contrast, atropine and scopolamine antagonize the effect of acetylcholine. The enzyme inhibition elicited by acetylcholine requires the presence of GTP, and disappears after intrastriatal injection of kainic acid. From these results, we infer that striatal adenylate cyclase can be modulated by muscarinic receptors.
乙酰胆碱可使从大鼠纹状体制备的纯化突触质膜的基础腺苷酸环化酶活性抑制30%-40%（半数有效浓度EC50 = 3微摩尔）。胆碱能受体激动剂抑制该环化酶活性的效力顺序如下：震颤素＞乙酰胆碱＞槟榔碱＞醋甲胆碱≥毒蕈碱≥卡巴胆碱＞氨甲酰甲胆碱。尼古丁不能抑制该环化酶，筒箭毒碱也不能抑制胆碱能药物的作用。相反，阿托品和东莨菪碱可拮抗乙酰胆碱的作用。乙酰胆碱引起的酶抑制作用需要鸟苷三磷酸（GTP）的存在，且在纹状体内注射 kainic 酸后消失。从这些结果我们推断，纹状体腺苷酸环化酶可受毒蕈碱受体调节。
