一种全新、简便且快速的全血中人类T淋巴细胞计数方法：大肠杆菌（美国典型培养物保藏中心11303）玫瑰花结试验。

A new, simple and rapid method for enumerating human T lymphocytes in full blood: the E. coli (ATCC 11303) rosette test.

作者信息

Orye E, Benoit Y, Roesbeke L, Van Yper M, De Wilde M

出版信息

J Immunol Methods. 1983 Jun 10;60(3):369-77. doi: 10.1016/0022-1759(83)90294-6.

Abstract

A new bacterial rosette technique for enumerating T lymphocytes is described. E. coli (strain B; ATCC 11303), fixed in formaldehyde after overnight growth in thioglycolate medium, are mixed with washed whole blood cells (100 microliters) and after incubation at 4 degrees C, slides are made, stained and counted. The nature of the lymphocytes forming E. coli rosettes was demonstrated by comparing their cytochemical staining characteristics with those of E rosetted lymphocytes, and by mixed E. coli and E, mouse E rosette and Fc receptor tests, and by mixed E. coli rosette tests and anti-Ig staining. E. coli and E rosette tests in controls and pediatric patients were also compared. The results show that T mu and T gamma cells rosette with E. coli.

摘要

本文描述了一种用于计数T淋巴细胞的新型细菌玫瑰花结技术。在硫乙醇酸盐培养基中过夜生长后用甲醛固定的大肠杆菌（菌株B；美国典型培养物保藏中心11303）与洗涤过的全血细胞（100微升）混合，在4℃孵育后制作玻片、染色并计数。通过将形成大肠杆菌玫瑰花结的淋巴细胞的细胞化学染色特征与E玫瑰花结淋巴细胞的特征进行比较，通过大肠杆菌与E、小鼠E玫瑰花结和Fc受体试验，以及通过混合大肠杆菌玫瑰花结试验和抗Ig染色，证明了形成大肠杆菌玫瑰花结的淋巴细胞的性质。还比较了对照组和儿科患者中的大肠杆菌和E玫瑰花结试验。结果表明，Tμ和Tγ细胞与大肠杆菌形成玫瑰花结。