Perry G W, Krayanek S R, Wilson D L
J Neurochem. 1983 Jun;40(6):1590-8. doi: 10.1111/j.1471-4159.1983.tb08131.x.
Damage to the sciatic nerve produces significant changes in the relative synthesis rates of some proteins in dorsal root ganglia and in the amounts of some fast axonally transported proteins in both the sciatic nerve and dorsal roots. We have now analyzed protein synthesis and axonal transport after cutting the other branch of dorsal root ganglia neurons, the dorsal roots. Two to three weeks after cutting the dorsal roots, [35S]methionine was used to label proteins in the dorsal root ganglia in vitro. Proteins synthesized in the dorsal root ganglia and transported along the sciatic nerve were analyzed on two-dimensional gels. All of the proteins previously observed to change after sciatic nerve damage were included in this study. No significant changes in proteins synthesized in dorsal root ganglia or rapidly transported along the sciatic nerve were detected. Axon regrowth from cut dorsal roots was observed by light and electron microscopy. Either the response to dorsal root damage is too small to be detected by our methods or changes in protein synthesis and fast axonal transport are not necessary for axon regrowth. When such changes do occur they may still aid in regrowth or be necessary for later stages in regeneration.
坐骨神经损伤会使背根神经节中某些蛋白质的相对合成速率以及坐骨神经和背根中某些快速轴突运输蛋白的量发生显著变化。我们现在分析了切断背根神经节神经元的另一分支——背根后蛋白质的合成和轴突运输情况。切断背根两到三周后，用[35S]甲硫氨酸在体外标记背根神经节中的蛋白质。在二维凝胶上分析背根神经节中合成并沿坐骨神经运输的蛋白质。本研究纳入了所有先前观察到的在坐骨神经损伤后发生变化的蛋白质。未检测到背根神经节中合成的蛋白质或沿坐骨神经快速运输的蛋白质有显著变化。通过光学显微镜和电子显微镜观察到了切断背根后的轴突再生。要么对背根损伤的反应太小以至于我们的方法检测不到，要么蛋白质合成和快速轴突运输的变化对于轴突再生不是必需的。当确实发生这种变化时，它们可能仍然有助于再生或对再生的后期阶段是必需的。
