信使核糖核酸前体的剪接受到针对小核核糖核蛋白的抗血清的抑制。

Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein.

作者信息

Padgett R A, Mount S M, Steitz J A, Sharp P A

出版信息

Cell. 1983 Nov;35(1):101-7. doi: 10.1016/0092-8674(83)90212-x.

PMID:6194895

Abstract

A mouse monoclonal antibody and human autoimmune sera directed against various classes of small ribonucleoprotein particles have been tested for inhibition of mRNA splicing in a soluble in vitro system. The splicing of the first and second leader exons of adenovirus late RNA was inhibited only by those sera that reacted with U1 RNP. Both U1 RNP-specific human autoimmune serum and sera directed against the Sm class of small nuclear RNPs, including a mouse monoclonal antibody, specifically inhibited splicing. Antisera specific for U2 RNP had no effect on splicing nor did antisera specific for the La or Ro class of small RNPs. These results suggest that U1 RNP is essential for the splicing of mRNA precursors.

摘要

针对各类小核糖核蛋白颗粒的小鼠单克隆抗体和人类自身免疫血清，已在一种可溶性体外系统中进行了抑制mRNA剪接的测试。腺病毒晚期RNA的第一和第二前导外显子的剪接仅被那些与U1核糖核蛋白反应的血清所抑制。U1核糖核蛋白特异性人类自身免疫血清以及针对小分子核核糖核蛋白Sm类的血清，包括一种小鼠单克隆抗体，均特异性地抑制剪接。U2核糖核蛋白特异性抗血清对剪接没有影响，针对La或Ro类小核糖核蛋白的抗血清也没有影响。这些结果表明，U1核糖核蛋白对于mRNA前体的剪接至关重要。