利用免疫细胞化学和酶细胞化学对里氏木霉中纤维素酶进行超微结构定位

Ultrastructural localization of cellulase in Trichoderma reesei using immunocytochemistry and enzyme cytochemistry.

作者信息

Chapman C M, Loewenberg J R, Schaller M J, Piechura J E

出版信息

J Histochem Cytochem. 1983 Dec;31(12):1363-6. doi: 10.1177/31.12.6195212.

DOI:10.1177/31.12.6195212

PMID:6195212

Abstract

Two components of the cellulase complex (E.C. 3.2.1.4) of the fungus Trichoderma reesei were localized at the ultrastructural level. Immunocytochemistry and enzyme cytochemistry demonstrated that cellobiohydrolase and beta-1,4 glucanase were localized within cisternae of endoplasmic reticulum and within membrane complexes of cellulose-grown hyphae. Both enzymes were also present in the culture medium. Glucose-grown control hyphae lacked enzyme-specific staining, and no enzyme activity was detected in the growth medium.

摘要

里氏木霉纤维素酶复合体（E.C. 3.2.1.4）的两个组分在超微结构水平上进行了定位。免疫细胞化学和酶细胞化学表明，纤维二糖水解酶和β-1,4葡聚糖酶定位于内质网的潴泡内以及纤维素生长菌丝的膜复合体中。这两种酶也存在于培养基中。以葡萄糖为生长底物的对照菌丝缺乏酶特异性染色，并且在生长培养基中未检测到酶活性。