Yen S H, Gaskin F, Fu S M
Am J Pathol. 1983 Dec;113(3):373-81.
A monoclonal antibody produced by a hybridoma between a plasmacytoma cell and a spleen cell from a mouse immunized with human brain microtubule fraction was demonstrated to stain neurofibrillary tangles of senile dementia of the Alzheimer type (SDAT). The antibody recognized at least 50% of the tangles in neuronal perikarya isolated from SDAT brains and stained a filamentous network in HeLa cells, fibroblasts, and astrocytes. It did not stain skin epithelial cells or neurons isolated from normal brains but reacted with Z bands in skeletal muscle. The monoclonal antibody stained coils in colchicine or colcemid-treated cultured cells in a pattern characteristic of 10-nm intermediate-sized filaments. Immunoblotting of Triton-insoluble cytoskeletal proteins of cultured cells electrophoresed in SDS polyacrylamide gels showed that the antigenic determinant is present in proteins of molecular weight 58,000 which comigrates with vimentin. Thus, it appears that the neurofibrillary tangles in SDAT share an antigenic determinant with vimentin.
一种由骨髓瘤细胞与用人类脑微管部分免疫的小鼠脾细胞杂交产生的单克隆抗体，被证明可对阿尔茨海默型老年痴呆症（SDAT）的神经原纤维缠结进行染色。该抗体识别出从SDAT脑部分离出的神经元胞体中至少50%的缠结，并对HeLa细胞、成纤维细胞和星形胶质细胞中的丝状网络进行染色。它不染色从正常脑部分离出的皮肤上皮细胞或神经元，但与骨骼肌中的Z带发生反应。该单克隆抗体以10纳米中间丝特有的模式对秋水仙碱或秋水仙酰胺处理的培养细胞中的线圈进行染色。在SDS聚丙烯酰胺凝胶中电泳的培养细胞的Triton不溶性细胞骨架蛋白的免疫印迹显示，抗原决定簇存在于分子量为58,000且与波形蛋白共迁移的蛋白质中。因此，看来SDAT中的神经原纤维缠结与波形蛋白共享一个抗原决定簇。
