15-羟基二十碳四烯酸（15-HETE）对抗免疫球蛋白E和钙离子载体诱导的人白细胞组胺释放的影响。与二十碳四炔酸和去甲二氢愈创木酸作用的比较。

Effect of 15-hydroxyeicosatetraenoic acid (15-HETE) on anti-immunoglobulin E- and calcium ionophore-induced histamine release from human leukocytes. Comparison with the effects of eicosatetraynoic acid and nordihydroguaiaretic acid.

作者信息

Dorsch W, Ring J, Riepel H

出版信息

Int Arch Allergy Appl Immunol. 1984;73(3):274-9. doi: 10.1159/000233481.

PMID:6199312

Abstract

15-Hydroxyeicosatetraenoic acid (15-HETE) was prepared by soybean lipoxygenase-mediated oxygenation of arachidonic acid to 15-hydroperoxyeicosatetraenoic acid (15-HETE) and subsequent reduction by NaBH4. 15-HETE was identified, purified and proved as biologically active by thin layer chromatography, high pressure liquid chromatography, gas chromatography/mass spectrometry and biological experiments on horse thrombocytes and rabbit peritoneal leukocytes. 15-HETE (0.1-40 microM) was added to peripheral leukocytes of 48 human donors (33 atopics, 15 nonatopics) which were challenged with rabbit anti-human-IgE or calcium ionophore A 23187. Its effect was compared with the effects of nordihydroguaiaretic acid (NDGA) and eicosatetraynoic acid (ETYA). NDGA and ETYA markedly inhibited histamine release (17 microM: 74 +/- 11 and 39 +/- 14,3%, respectively), whereas 15-HETE neither stimulated nor inhibited spontaneous anti-IgE- or calcium ionophore A 23187-induced histamine release.

摘要

15-羟基二十碳四烯酸（15-HETE）通过大豆脂氧合酶介导的花生四烯酸氧化为15-氢过氧二十碳四烯酸（15-HPETE），随后用硼氢化钠还原而制备。通过薄层色谱、高压液相色谱、气相色谱/质谱以及对马血小板和兔腹腔白细胞进行的生物学实验，对15-HETE进行了鉴定、纯化并证明其具有生物活性。将15-HETE（0.1 - 40微摩尔）添加到48名人类供体（33名特应性个体、15名非特应性个体）的外周血白细胞中，这些白细胞用兔抗人IgE或钙离子载体A 23187进行刺激。将其效果与去甲二氢愈创木酸（NDGA）和二十碳四炔酸（ETYA）的效果进行比较。NDGA和ETYA显著抑制组胺释放（17微摩尔时分别为74±11和39±14.3%），而15-HETE既不刺激也不抑制自发的抗IgE或钙离子载体A 23187诱导的组胺释放。