使用抗白细胞抗体和在 Percoll 上进行密度梯度离心法分离豚鼠嗜碱性粒细胞。

Isolation of guinea pig basophils using anti-leukocyte antibody and density gradient centrifugation on Percoll.

作者信息

Graziano F M, Lipham W, Swaminathan N, Brendel J, Graziano F M, Warner T

出版信息

J Immunol Methods. 1984 Feb 24;67(1):157-65. doi: 10.1016/0022-1759(84)90094-2.

PMID:6199429

Abstract

Peripheral blood basophils were isolated from guinea pigs using a 2-step procedure. Initial enrichment of basophils was achieved by treating leukocyte preparations obtained from whole blood with antibody to contaminating granulocytes and mononuclear cells. Basophil populations of 82% purity (mean; range 65-99%) were obtained by subsequent application of the antibody treated preparation to discontinuous Percoll gradients. The isolated basophils were viable and shown to be functional by histamine release upon stimulation with various secretogogues.

摘要

采用两步法从豚鼠中分离外周血嗜碱性粒细胞。通过用针对污染性粒细胞和单核细胞的抗体处理全血获得的白细胞制剂，实现嗜碱性粒细胞的初步富集。随后将抗体处理过的制剂应用于不连续的 Percoll 梯度，获得纯度为 82%（平均值；范围 65 - 99%）的嗜碱性粒细胞群体。分离出的嗜碱性粒细胞具有活性，并且在用各种促分泌剂刺激后通过组胺释放显示其具有功能。