Zirkin B R, Strandberg J D
Anat Rec. 1984 Feb;208(2):207-14. doi: 10.1002/ar.1092080207.
Stereological methods at the light and electron microscopic levels were used to examine the morphological changes in the beagle prostate associated with increasing age and with the occurrence of spontaneous benign hyperplasia. Analyses of semithin sections of tissue embedded in plastic revealed that proliferation of prostatic glandular and stromal components, increase in epithelial cell numbers, and increase in epithelial cell size all contributed to the dramatic increase in prostate weight seen in aging dogs. Analyses at the electron microscopic level revealed that the volume densities of rough endoplasmic reticulum and secretion granules were significantly lower in epithelial cells of the immature prostate than in the prostate of mature dogs (greater than 1.5 years of age). In contrast, no significant differences were seen in the volume densities of rough endoplasmic reticulum, free ribosomes, secretion granules, smooth endoplasmic reticulum, or mitochondria in randomly selected prostatic epithelial cells of dogs 1.5 to 9 years of age, whether or not the prostates were hyperplastic.
运用光镜和电镜水平的体视学方法，研究比格犬前列腺随年龄增长及自发性良性增生发生时的形态学变化。对包埋于塑料中的组织半薄切片分析显示，前列腺腺上皮和基质成分的增殖、上皮细胞数量的增加以及上皮细胞大小的增大，均导致老龄犬前列腺重量显著增加。电镜水平分析显示，未成熟前列腺上皮细胞中粗面内质网和分泌颗粒的体积密度显著低于成年犬（年龄大于1.5岁）的前列腺。相比之下，在1.5至9岁犬的随机选取的前列腺上皮细胞中，无论前列腺是否增生，粗面内质网、游离核糖体、分泌颗粒、滑面内质网或线粒体的体积密度均无显著差异。
