Southern J A, Katz W, Woods D R
Antimicrob Agents Chemother. 1984 Feb;25(2):253-7. doi: 10.1128/AAC.25.2.253.
A cell-bound bacteriocin was extracted from a Bacteroides fragilis BF-11 strain by treating the cells with a low-molarity buffer (0.01 M Tris-hydrochloride, pH 8.0). Sucrose osmotic shock experiments and ultrasonic lysis of whole cells indicated that the majority of the bacteriocin was located at the cell surface. Culture supernatants contained no significant bacteriocin activity. The bacteriocin was purified by DEAE-cellulose and Sephacryl S200 chromatography and had an apparent molecular weight of approximately 7,000. It was relatively heat stable and was inactivated by proteases. There was a delay of approximately 3.5 h before DNA, RNA, and protein synthesis were inhibited by the bacteriocin. Inhibition of macromolecular synthesis coincided with lysis of the susceptible indicator strain.
通过用低摩尔浓度缓冲液（0.01 M 三羟甲基氨基甲烷盐酸盐，pH 8.0）处理细胞，从脆弱拟杆菌BF - 11菌株中提取出一种细胞结合型细菌素。蔗糖渗透休克实验和全细胞超声裂解表明，大部分细菌素位于细胞表面。培养上清液中未检测到显著的细菌素活性。该细菌素通过DEAE - 纤维素和Sephacryl S200柱层析进行纯化，其表观分子量约为7000。它相对耐热，可被蛋白酶灭活。在细菌素抑制DNA、RNA和蛋白质合成之前，大约有3.5小时的延迟。大分子合成的抑制与敏感指示菌株的裂解同时发生。
