单克隆抗体可保护小鼠免受呼吸道合胞病毒感染。
Monoclonal antibodies protect against respiratory syncytial virus infection in mice.
作者信息
Taylor G, Stott E J, Bew M, Fernie B F, Cote P J, Collins A P, Hughes M, Jebbett J
出版信息
Immunology. 1984 May;52(1):137-42.
Abstract
Twenty-five monoclonal antibodies (Mab) to respiratory syncytial virus (RSV) and two to hepatitis B virus were inoculated intravenously into mice. Twenty-four hours later the mice were challenged intranasally with RSV. Eleven of 14 Mab against fusion protein and four out of six Mab against a larger glycoprotein (GP84) significantly reduced the titre of RSV in the lungs when mice were killed 5 days later. Five Mab against three other RSV proteins and two Mab against hepatitis B virus had no significant effect on RSV infection. These results indicated that serum IgG against one epitope on the fusion protein and another on the larger glycoprotein (GP84) will completely protect mice against challenge. These epitopes are primary candidates for an RSV vaccine produced by techniques of gene cloning and peptide synthesis.
摘要
将25种针对呼吸道合胞病毒（RSV）的单克隆抗体（Mab）以及2种针对乙型肝炎病毒的单克隆抗体静脉注射到小鼠体内。24小时后，对小鼠进行鼻内RSV攻击。5天后处死小鼠时，14种针对融合蛋白的单克隆抗体中有11种以及6种针对一种较大糖蛋白（GP84）的单克隆抗体中有4种显著降低了肺内RSV的滴度。5种针对其他3种RSV蛋白的单克隆抗体以及2种针对乙型肝炎病毒的单克隆抗体对RSV感染无显著影响。这些结果表明，针对融合蛋白上一个表位以及较大糖蛋白（GP84）上另一个表位的血清IgG可完全保护小鼠免受攻击。这些表位是通过基因克隆和肽合成技术生产RSV疫苗的主要候选对象。
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