Mesquita-Guimarães J, Mesquita-Guimarães I
J Submicrosc Cytol. 1984 Apr;16(2):387-94.
In order to obtain information about the mechanism of dyskeratosis in Darier's disease, we have examined skin sections at different steps of differentiation at light and electron microscopic level in three patients. Corps ronds occurred at the basal aspect of spinous layer, while grains were abundant in the bulla and in the granular layer of the peripheral epidermis. Corps ronds contained cytoplasmic vacuoles and great number of keratinosomes. Their cytoplasm was divided by a circular ring of tonofilaments into a juxtanuclear and a peripheral compartment. More superficially corps ronds became dyskeratotic keratohyalin-containing cells by acquiring increasing amounts of keratohyalin granules; the cytoplasm turned more vacuolated and poor in keratinosomes. Grains exhibited nuclear pyknosis, shrinkage and densification of mitochondria, numerous tonofilament bundles and confluent vacuoles after disruption of their membrane. In these cells, keratohyalin granules appeared less electrondense and keratinosomes were rare or absent. Horny cells contained empty areas resulting from the persistence of vacuolar spaces. These observations suggest that corps ronds and grains represent stages in the same successive differentiation line and that grains give rise to the changed horny cells occurring in this condition.
为了获取毛囊角化病中角化不良机制的相关信息，我们在光学显微镜和电子显微镜水平上，对三名患者不同分化阶段的皮肤切片进行了检查。圆体细胞出现在棘层的基底部，而颗粒细胞在水疱和外周表皮的颗粒层中大量存在。圆体细胞含有细胞质空泡和大量角蛋白小体。它们的细胞质被一圈张力丝分成核周区和外周区。在更浅表的部位，圆体细胞通过获得越来越多的透明角质颗粒而变成含有角化不良透明角质的细胞；细胞质变得更加空泡化，角蛋白小体减少。颗粒细胞表现出核固缩、线粒体收缩和致密化、大量张力丝束以及膜破裂后融合的空泡。在这些细胞中，透明角质颗粒的电子密度较低，角蛋白小体很少或不存在。角质形成细胞含有因空泡间隙持续存在而产生的空洞区域。这些观察结果表明，圆体细胞和颗粒细胞代表了同一连续分化过程中的不同阶段，并且颗粒细胞会导致在这种情况下出现的角质形成细胞变化。
