Roots B I, Agrawal D, Weir G, Agrawal H C
J Neurochem. 1984 Nov;43(5):1421-4. doi: 10.1111/j.1471-4159.1984.tb05403.x.
Myelin isolated from goldfish brain shows a multilamellar structure with a major dense line and two intraperiod lines. Sodium dodecyl sulfate gel electrophoresis revealed that the protein profile of goldfish brain myelin is distinctly different from that of rat brain myelin. No protein migrating to the position of proteolipid protein or DM-20 was seen in goldfish myelin. Goldfish acclimated to 5 degrees, 15 degrees, and 30 degrees C showed no qualitative differences in myelin proteins. The 13.5 kD protein in goldfish brain myelin and brain homogenate was intensely immunostained with the antiserum to human basic protein by the immunoblot technique. In contrast, none of the proteins of goldfish myelin were immunostained with antiproteolipid protein serum; however, both proteolipid protein and DM-20 of rat brain myelin were immunostained. The significance of the synthesis of myelin proteins by astrocytes in the goldfish brain is discussed.
从金鱼脑部分离出的髓磷脂呈现出一种具有一条主要致密线和两条周期内线的多层结构。十二烷基硫酸钠凝胶电泳显示，金鱼脑髓磷脂的蛋白质谱与大鼠脑髓磷脂的明显不同。在金鱼髓磷脂中未观察到迁移至蛋白脂蛋白或DM - 20位置的蛋白质。适应5摄氏度、15摄氏度和30摄氏度的金鱼在髓磷脂蛋白方面未表现出定性差异。通过免疫印迹技术，金鱼脑髓磷脂和脑匀浆中的13.5 kD蛋白质被抗人碱性蛋白抗血清强烈免疫染色。相比之下，金鱼髓磷脂的蛋白质均未被抗蛋白脂蛋白血清免疫染色；然而，大鼠脑髓磷脂的蛋白脂蛋白和DM - 20均被免疫染色。本文讨论了金鱼脑星形胶质细胞合成髓磷脂蛋白的意义。
