Zimmerman B, Swain S L, Dutton R W
J Immunol. 1982 Aug;129(2):515-20.
The effect of anti-thymocyte serum (ATS) on functional T cell subsets within the immunoregulatory network was examined. We found that low doses of ATS depleted the IL 2-producing subset of T helper cells and higher doses of antiserum also eliminated cytotoxic precursors. It was estimated that a standard low dose of ATS, which reduced T helper cells 80-fold, depleted cytotoxic precursors only about four-fold. The data indicate that ATS acts on different T cell subsets in a dose-dependent fashion and suggest that monoclonal antibodies might be used to preferentially deplete specific T cell subpopulations. Two monoclonal rat anti-mouse Lyt sera, however, proved ineffective in inducing functional changes in vivo, although an alloantiserum to Lyt1.1 was able to reduce cell-mediated cytotoxicity in a dose-dependent fashion. The results support the feasibility of specific depletion of T cell subsets with anti-lymphoid antibodies.
研究了抗胸腺细胞血清（ATS）对免疫调节网络内功能性T细胞亚群的影响。我们发现，低剂量的ATS可耗尽产生白细胞介素2的辅助性T细胞亚群，而高剂量的抗血清也可消除细胞毒性前体细胞。据估计，标准低剂量的ATS可使辅助性T细胞减少80倍，而细胞毒性前体细胞仅减少约4倍。数据表明，ATS以剂量依赖的方式作用于不同的T细胞亚群，并提示单克隆抗体可用于优先耗尽特定的T细胞亚群。然而，两种单克隆大鼠抗小鼠Lyt血清在体内诱导功能变化方面无效，尽管针对Lyt1.1的同种抗血清能够以剂量依赖的方式降低细胞介导的细胞毒性。这些结果支持了用抗淋巴细胞抗体特异性耗尽T细胞亚群的可行性。
