Mayahara T, Kamimura T, Tanaka M, Shibanoki S, Tokuyoshi K, Maruyama I, Yamada S
Physiol Bohemoslov. 1982;31(4):297-303.
In order to investigate the mechanism of skeletal muscle relaxation induced by dimethyl sulfoxide, 2-butoxyethanol and dimethyl sulfoxide were examined for their effects on 1) Ca2+ uptake into and efflux from sarcoplasmic reticulum vesicles prepared from rabbit fast skeletal muscle and crayfish tail muscle by the murexide method, 2) ATPase activities of rabbit reticulum vesicles, 3) the isolated phrenic nerve-diaphragm preparation of the rat and 4) crayfish opener muscle preparation. Ca2+ efflux rate from rabbit reticulum vesicles was markedly decreased with increasing concentrations (5-20% v/v) of dimethyl sulfoxide without affecting the maximum Ca2+ uptake by the reticulum. 2-Butoxyethanol showed quite contrary effects. Dimethyl sulfoxide strongly inhibited the activity of basal ATPase rather than of Ca2+-dependent ATPase. 2-Butoxyethanol did not significantly inhibit the activity of basal ATPase, but markedly increased Ca2+-dependent ATPase activity. Antagonisms between dimethyl sulfoxide and caffeine were demonstrated either in contractions of crayfish opener muscles or in the Ca2+ release from crayfish sarcoplasmic reticulum vesicles. These results indicate a possibility that dimethyl sulfoxide reversibly induces skeletal muscle relaxation mainly in the sarcoplasmic reticulum by means of decreasing the rate and the amount of Ca2+ release from the reticulum.
为了研究二甲基亚砜诱导骨骼肌松弛的机制，检测了2-丁氧基乙醇和二甲基亚砜对以下方面的影响：1）采用紫脲酸铵法从兔快肌和小龙虾尾肌制备的肌浆网囊泡对Ca2+的摄取和释放；2）兔网织体囊泡的ATP酶活性；3）大鼠膈神经-膈肌离体标本；4）小龙虾开肌标本。随着二甲基亚砜浓度（5-20% v/v）的增加，兔网织体囊泡的Ca2+释放速率显著降低，而不影响网织体对Ca2+的最大摄取量。2-丁氧基乙醇表现出相反的作用。二甲基亚砜强烈抑制基础ATP酶的活性，而不是Ca2+依赖性ATP酶的活性。2-丁氧基乙醇对基础ATP酶的活性没有显著抑制作用，但显著增加了Ca2+依赖性ATP酶的活性。在小龙虾开肌收缩或小龙虾肌浆网囊泡的Ca2+释放中，均证实了二甲基亚砜与咖啡因之间的拮抗作用。这些结果表明，二甲基亚砜可能通过降低肌浆网Ca2+释放的速率和量，主要在肌浆网中可逆地诱导骨骼肌松弛。
