巯基化合物在粗糙脉孢菌酪氨酸酶活性调控中的作用

Role of sulfhydryl compounds in the control of tyrosinase activity in Neurospora crassa.

作者信息

Prade R A, Terenzi H F

出版信息

Biochem Genet. 1982 Dec;20(11-12):1235-43. doi: 10.1007/BF00498946.

PMID:6219663

Abstract

It is known that Neurospora crassa mycelia cultured in standard concentrations (76 to 190 micrograms/ml) of sulfate accumulate a low molecular weight inhibitor of tyrosinase (monophenol, dihydroxyphenylalanine: oxygen oxidoreductase; EC 1.14.1.18.1.). This is not observed in cultures grown under sulfate-limiting conditions. The chemical nature of tyrosinase inhibition was investigated. It was shown to be due to the low molecular weight sulfhydryl fraction of the extracts, in which glutathione is predominant. The concentration of low molecular weight sulfhydryl compounds decreased sharply in mycelia submitted to various treatments which also derepressed tyrosinase, such as (i) starvation in phosphate buffer, (ii) treatment with cycloheximide, and (iii) mating. These results suggest that the concentration of sulfhydryl compounds may be of physiological significance in the control of tyrosinase activity in N. crassa.

摘要

已知在标准浓度（76至190微克/毫升）的硫酸盐中培养的粗糙脉孢菌菌丝体会积累一种低分子量的酪氨酸酶抑制剂（单酚，二羟基苯丙氨酸：氧氧化还原酶；EC 1.14.1.18.1）。在硫酸盐限制条件下生长的培养物中未观察到这种情况。对酪氨酸酶抑制的化学性质进行了研究。结果表明，这是由于提取物中低分子量的巯基部分所致，其中谷胱甘肽占主导。在经过各种也会解除酪氨酸酶抑制的处理的菌丝体中，低分子量巯基化合物的浓度急剧下降，例如（i）在磷酸盐缓冲液中饥饿，（ii）用环己酰亚胺处理，以及（iii）交配。这些结果表明，巯基化合物的浓度在粗糙脉孢菌酪氨酸酶活性的控制中可能具有生理意义。