Lyt phenotype of lymphocytes producing murine macrophage chemotactic lymphokine.

Normal or alloantigen (irradiated C57BL/6 mouse spleen cells)-immunized C3H/HeN mouse spleen cells, and dinitrophenyl ovalbumin (DNP-OA)-immunized C3H/HeN mouse lymph node cells were tested after treatment with anti-Thy-1.2, anti-Lyt-1.1, or anti-Lyt-2.1 antibody in the presence of complement for their ability to produce macrophage chemotactic lymphokine in response to stimulation with either mitogen (concanavalin A) or specific antigens. When normal spleen cells were stimulated with Con A or immunized cells were stimulated with specific antigens, it was found that the Lyt-1+2-T-cell subpopulation was primarily responsible for the macrophage chemotactic lymphokine production by T cells.