[自旋标记在高胆固醇血症家兔骨骼肌肌浆网钙-ATP酶分析中的应用]

[Spin labels in the analysis of Ca-ATPase in the sarcoplasmic reticulum of rabbit skeletal muscles in hypercholesteremia].

作者信息

Timofeev A A, Stoĭda L V, Azizova O A, Maksina A G, Chernysheva G V

出版信息

Biull Eksp Biol Med. 1983 Nov;96(11):59-62.

Abstract

The method of electron paramagnetic resonance with spin-labeled maleimide was used to study variation of the structure of Ca-ATPase of the sarcoplasmic reticulum (SR) in rabbit skeletal muscles under long-term hypercholesterolemia (HC). The rate of the maleimide spin label binding with Ca-ATPase of the SR was decreased in HC, which correlated with a lesser access of spin-labeled thiol groups for potassium ferricyanide and sodium ascorbate. HC led to a considerable reduction in the lability and to enhancement of hydrophobia of the spin-labeled fragment of the enzyme. It is concluded that the disordered function of the SR Ca-pump is a consequence of structural changes in the Ca-ATPase molecule in HC.

摘要

采用自旋标记马来酰亚胺的电子顺磁共振方法，研究长期高胆固醇血症（HC）状态下兔骨骼肌肌浆网（SR）钙 - ATP酶结构的变化。在高胆固醇血症中，马来酰亚胺自旋标记与肌浆网钙 - ATP酶的结合速率降低，这与自旋标记的巯基对铁氰化钾和抗坏血酸钠的可及性降低相关。高胆固醇血症导致该酶自旋标记片段的稳定性显著降低以及疏水性增强。得出结论：肌浆网钙泵功能紊乱是高胆固醇血症中钙 - ATP酶分子结构变化的结果。

相似文献

1

[Spin labels in the analysis of Ca-ATPase in the sarcoplasmic reticulum of rabbit skeletal muscles in hypercholesteremia].[自旋标记在高胆固醇血症家兔骨骼肌肌浆网钙-ATP酶分析中的应用]

Biull Eksp Biol Med. 1983 Nov;96(11):59-62.

2

[Study of Ca-dependent ATPase of sarcoplasmic reticulum by the use of selectively bound spin labels].[利用选择性结合的自旋标记研究肌浆网钙依赖性ATP酶]

Mol Biol (Mosk). 1981 May-Jun;15(3):668-79.

3

Phosphorylation of phospholamban by cAMP-dependent protein kinase enhances interactions between Ca-ATPase polypeptide chains in cardiac sarcoplasmic reticulum membranes.环磷酸腺苷依赖性蛋白激酶对受磷蛋白的磷酸化作用增强了心肌肌浆网膜中钙-ATP酶多肽链之间的相互作用。

Biochemistry. 1996 Sep 3;35(35):11247-59. doi: 10.1021/bi960864q.

4

[Role of lipid peroxidation in changes in the structure of Ca-ATPase in skeletal muscle sarcoplasmic reticulum during hypercholesterolemia].[脂质过氧化在高胆固醇血症期间骨骼肌肌浆网中钙 -ATP 酶结构变化中的作用]

Biull Eksp Biol Med. 1985 Mar;99(3):301-3.

5

[Lipid peroxidation as a major factor in the modification of the catalytic function of Ca-ATPase of the sarcoplasmic reticulum in hypercholesterolemia].

Biull Eksp Biol Med. 1987 Apr;103(4):415-8.

6

[Interaction of spin-labelled phosphatidyl choline with Ca-dependent ATPase from sarcoplasmic reticulum].[自旋标记磷脂酰胆碱与肌浆网钙依赖性ATP酶的相互作用]

Biokhimiia. 1982 Dec;47(12):2042-7.

7

[Effect of cholesterol on the cooperativeness of the Ca-ATPase of the sarcoplasmic reticulum in rabbit skeletal muscle].

Biull Eksp Biol Med. 1984 Dec;98(12):671-3.

8

[Change in the molecular properties of sarcoplasmic reticulum Ca-ATPase during modification of the membranes with cholesterol].

Biull Eksp Biol Med. 1983 Oct;96(10):38-40.

9

Effect of temperature on Ca-ATPase from sarcoplasmic reticulum membranes: ESR studies.温度对肌质网膜钙-ATP酶的影响：电子自旋共振研究

Gen Physiol Biophys. 1986 Oct;5(5):551-61.

10

Electron paramagnetic resonance reveals a large-scale conformational change in the cytoplasmic domain of phospholamban upon binding to the sarcoplasmic reticulum Ca-ATPase.电子顺磁共振显示，受磷蛋白与肌浆网钙ATP酶结合的影响，其胞质结构域发生了大规模构象变化。

Biochemistry. 2004 May 18;43(19):5842-52. doi: 10.1021/bi035749b.

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