Timofeev A A, Stoĭda L V, Azizova O A, Maksina A G, Chernysheva G V
Biull Eksp Biol Med. 1983 Nov;96(11):59-62.
The method of electron paramagnetic resonance with spin-labeled maleimide was used to study variation of the structure of Ca-ATPase of the sarcoplasmic reticulum (SR) in rabbit skeletal muscles under long-term hypercholesterolemia (HC). The rate of the maleimide spin label binding with Ca-ATPase of the SR was decreased in HC, which correlated with a lesser access of spin-labeled thiol groups for potassium ferricyanide and sodium ascorbate. HC led to a considerable reduction in the lability and to enhancement of hydrophobia of the spin-labeled fragment of the enzyme. It is concluded that the disordered function of the SR Ca-pump is a consequence of structural changes in the Ca-ATPase molecule in HC.
采用自旋标记马来酰亚胺的电子顺磁共振方法，研究长期高胆固醇血症（HC）状态下兔骨骼肌肌浆网（SR）钙 - ATP酶结构的变化。在高胆固醇血症中，马来酰亚胺自旋标记与肌浆网钙 - ATP酶的结合速率降低，这与自旋标记的巯基对铁氰化钾和抗坏血酸钠的可及性降低相关。高胆固醇血症导致该酶自旋标记片段的稳定性显著降低以及疏水性增强。得出结论：肌浆网钙泵功能紊乱是高胆固醇血症中钙 - ATP酶分子结构变化的结果。
