Porwit-Ksiazek A, Aman P, Ksiazek T, Biberfeld P
Scand J Immunol. 1983 Dec;18(6):495-9. doi: 10.1111/j.1365-3083.1983.tb00883.x.
In the present study, combined methods (indirect immunofluorescence with monoclonal antibodies, Percoll density fractionation, FACS analysis, and the cytotoxicity test) were used for further characterization of peripheral blood Leu 7+ cells (human NK and K cells). The Leu 7+ cell content was found to be relatively higher in the low-density cell fraction in which cells of large granular lymphocyte morphology predominated. However, Leu 7+ cells were also present in intermediate and high-density fractions. Low-density Leu 7+ cells were characterized by both Leu 2 (T suppressor/cytotoxic) and OKM1 (myelomonocytic) markers, whereas among high-density Leu 7+ cells the Leu 2 phenotype strictly predominated. Depletion of OKT3+ cells from the non-adherent cell population caused a decrease of cells with T helper and T suppressor phenotypes but did not have this effect on Leu 7+ and OKM1+ cells. After depletion of Leu 7+ cells from the OKT3- population the content of both T suppressor and OKM1+ cells decreased. Both the present results and previous reports enable us to conclude that two main Leu 7+ cell subpopulations are present in blood, namely Leu 7+Leu 2+/Leu 4+ and Leu 7+/OKM1+ cells. The presence of small and large Leu 7+ cells was also shown by FACS analysis.
在本研究中，联合方法（使用单克隆抗体的间接免疫荧光法、Percoll密度梯度离心法、流式细胞术分析和细胞毒性试验）被用于进一步鉴定外周血Leu 7+细胞（人自然杀伤细胞和杀伤细胞）。发现低密度细胞组分中Leu 7+细胞含量相对较高，其中以大颗粒淋巴细胞形态的细胞为主。然而，Leu 7+细胞也存在于中密度和高密度组分中。低密度Leu 7+细胞同时具有Leu 2（T抑制/细胞毒性）和OKM1（骨髓单核细胞）标志物特征，而在高密度Leu 7+细胞中，Leu 2表型占绝对优势。从非贴壁细胞群体中去除OKT3+细胞导致具有T辅助和T抑制表型的细胞减少，但对Leu 7+和OKM1+细胞没有这种影响。从OKT3-群体中去除Leu 7+细胞后，T抑制细胞和OKM1+细胞的含量均下降。目前的结果和先前的报道均使我们能够得出结论，血液中存在两个主要的Leu 7+细胞亚群，即Leu 7+Leu 2+/Leu 4+和Leu 7+/OKM1+细胞。流式细胞术分析也显示了小和大的Leu 7+细胞的存在。
