Bergmann K C, Waldman R H, Stone J
Allerg Immunol (Leipz). 1983;29(4):204-8.
The percentage of lymphocyte subpopulations from the bronchoalveolar space of Balb/c-mice expressing B-(Ia), and T-(Thy 1.2, Lyt 1.2 and Lyt 2.2) cell markers was determined using monoclonal antibodies and complement in the direct cytotoxic test. The cells were obtained by lung lavage from unstimulated animals. Only 6% of total cell yield (1.1 +/- 0.8 X 10(5) cells/mouse) showed no adherence and were separated by density centrifugation. The majority (95%) of these cells were esterase-negative lymphocytes. The mice had a higher percentage of B cells (51%) and suppressor cells (14%) and a lower percentage of total T cells (45%) and helper cells (28%) in lavage fluid of lungs than in the blood.
使用单克隆抗体和补体在直接细胞毒性试验中测定了来自Balb/c小鼠支气管肺泡腔表达B-（Ia）和T-（Thy 1.2、Lyt 1.2和Lyt 2.2）细胞标志物的淋巴细胞亚群的百分比。细胞通过对未受刺激动物进行肺灌洗获得。总细胞产量（1.1±0.8×10⁵个细胞/小鼠）中只有6%的细胞不贴壁，通过密度离心分离。这些细胞中的大多数（95%）是酯酶阴性淋巴细胞。与血液相比，小鼠肺灌洗液中的B细胞百分比（51%）和抑制细胞百分比（14%）更高，总T细胞百分比（45%）和辅助细胞百分比（28%）更低。
