Marks S C
Am J Anat. 1978 Jan;151(1):119-29. doi: 10.1002/aja.1001510110.
After ia (osteopetrotic) rats receive whole body radiation and an injection of spleen cells from a normal littermate, the dense, sclerotic skeleton characteristic of osteopetrosis is rapidly remodeled and becomes normal in appearance radiographically and histologically within three weeks. The mechanism of this skeletal transformation has been explored in cured ia rats by light and electron microscopic examination of osteoclasts. In ia rats less than 25 days of age, osteoclasts viewed by electron microscopy lack a ruffled border - the extensive elaboration of plasma membrane next to the bone surface. Cured ia rats have osteoclasts with ruffled borders indistinguishable from those of normal littermates. In ia rats that receive only 600 rads whole body radiation, osteoclasts are still present three weeks later, but appear abnormal by light microscopy, with dense nuclei and lacking cytoplasmic vacuoles next to the bone surface. Cured ia rats have two types of osteoclasts, one type indistinguishable from osteoclasts of normal littermates by light microscopy, the other resembling osteoclasts of ia rats that received radiation only. These data indicate that the mechanism of the spleen cell cure for osteopetrosis in ia rats is rapid remodeling of the skeleton produced by osteoclasts with ruffled borders. Whether normal spleen cells produce these osteoclasts directly by cell division or indirectly by elaboration of some unknown local factor required for formations of ruffled borders by ia osteoclasts is not known.
患有骨质石化症（ia）的大鼠接受全身辐射并注射来自正常同窝幼仔的脾细胞后，骨质石化症特有的致密硬化骨骼会迅速重塑，并在三周内其外观在放射学和组织学上变得正常。通过对破骨细胞进行光镜和电镜检查，在治愈的ia大鼠中探索了这种骨骼转变的机制。在小于25日龄的ia大鼠中，电镜观察到的破骨细胞缺乏皱襞缘——紧邻骨表面的质膜广泛延伸。治愈的ia大鼠具有与正常同窝幼仔难以区分的带有皱襞缘的破骨细胞。在仅接受600拉德全身辐射的ia大鼠中，三周后破骨细胞仍然存在，但通过光镜观察显得异常，细胞核致密，且在骨表面缺乏细胞质空泡。治愈的ia大鼠有两种类型的破骨细胞，一种通过光镜观察与正常同窝幼仔的破骨细胞难以区分，另一种类似于仅接受辐射的ia大鼠的破骨细胞。这些数据表明，ia大鼠中脾细胞治愈骨质石化症的机制是由带有皱襞缘的破骨细胞对骨骼进行快速重塑。正常脾细胞是直接通过细胞分裂产生这些破骨细胞，还是通过产生ia破骨细胞形成皱襞缘所需的某种未知局部因子间接产生，目前尚不清楚。
