Tibayrenc M, Echalar L, Dujardin J P, Poch O, Desjeux P
Trans R Soc Trop Med Hyg. 1984;78(4):519-25. doi: 10.1016/0035-9203(84)90075-0.
132 Trypanosoma cruzi stocks were collected in southern Bolivia (99 stocks in Tupiza, 33 in Tarija), and were characterized using five enzymes (six loci). From these 132 stocks, a sample of 21 was studied using 10 enzymes (12 loci) to establish the genetic distances between them. Only five different isoenzymic strains were registered among the 132 stocks: the taxonomic status of these strains is discussed. The distribution of the strains indicated that a Founder effect was not a constant fact at the level of the house and of the suburb, but that a Founder effect was more apparent for greater geographical distances. All strains were transmitted sympatrically by the same vector Triatoma infestans. Genotype frequencies demonstrated the lack of Mendelian sexuality among stocks of T. cruzi from southern Bolivia, confirming our previous results.
132株克氏锥虫样本采自玻利维亚南部（图皮萨99株，塔里哈33株），并使用5种酶（6个位点）进行特征分析。从这132株样本中选取21株，使用10种酶（12个位点）进行研究，以确定它们之间的遗传距离。在这132株样本中仅记录到5种不同的同工酶菌株：对这些菌株的分类地位进行了讨论。菌株的分布表明，奠基者效应在家庭和郊区层面并非恒定现象，但在更大地理距离上，奠基者效应更为明显。所有菌株均由同一种传播媒介——侵扰锥猎蝽进行同域传播。基因型频率表明，玻利维亚南部克氏锥虫菌株缺乏孟德尔式有性生殖，这证实了我们之前的结果。
