Jaye M, Wu F S, Lucas-Lenard J M
Biochim Biophys Acta. 1980;606(1):1-12. doi: 10.1016/0005-2787(80)90092-1.
The effect of infection of mouse L cells by vesicular stomatitis virus on the synthesis of ribosomal proteins was investigated using two-dimensional polyacrylamide gel electrophoresis to analyze the ribosomal proteins. It was found that the synthesis of nearly all of the cytoplasmic ribosomal proteins examined was inhibited by infection and mostly to the same extent. Analysis of the ribosomal proteins extracted from intact ribosomes indicated that infection also reduces the incorporation of all the ribosomal proteins tested into assembled ribosomes. The inhibition of ribosome assembly was greater than the inhibition of synthesis of ribosomal proteins, suggesting that some other factor was also limiting the assembly of ribosomes. As shown in this report, infection also inhibits ribosomal RNA production. Thus, the decreased assembly of ribosomes in infected cells probably results from the inhibition of synthesis of both ribosomal proteins and ribosomal RNA.
利用二维聚丙烯酰胺凝胶电泳分析核糖体蛋白，研究了水泡性口炎病毒感染小鼠L细胞对核糖体蛋白合成的影响。结果发现，几乎所有检测的细胞质核糖体蛋白的合成均受到感染抑制，且大多受到相同程度的抑制。对从完整核糖体中提取的核糖体蛋白的分析表明，感染还减少了所有测试核糖体蛋白掺入组装核糖体中的量。核糖体组装的抑制作用大于核糖体蛋白合成的抑制作用，这表明还有其他因素也在限制核糖体的组装。如本报告所示，感染还会抑制核糖体RNA的产生。因此，受感染细胞中核糖体组装减少可能是由于核糖体蛋白和核糖体RNA合成均受到抑制所致。
