Rodríguez N, Renaud F L
J Cell Biol. 1980 May;85(2):242-7. doi: 10.1083/jcb.85.2.242.
A study was made of the interrelationship of serotonin, cAMP, and calcium ions in the regulation of regeneration of cilia by Tetrahymena pyriformis. All these compounds stimulated the regeneration, whereas a blocker of serotonin synthesis, p-chlorophenylalanine, and a calcium chelator, EGTA, inhibited the process. This inhibition could be overcome by the addition of any of the stimulatory compounds. cAMP was also found to be inhibitory at high concentrations. The intracellular concentration of this nucleotide was found to increase during the regeneration, and this increase occurred precociously in the presence of serotonin. It was concluded that serotonin may regulate ciliary regeneration by a mechanism involving cAMP And calcium ions, but that the causal relationships among these compounds still need to be established.
对梨形四膜虫纤毛再生调节过程中血清素、环磷酸腺苷(cAMP)和钙离子的相互关系进行了研究。所有这些化合物都能刺激再生,而血清素合成阻滞剂对氯苯丙氨酸和钙螯合剂乙二醇双四乙酸(EGTA)则抑制这一过程。添加任何一种刺激性化合物都可以克服这种抑制作用。还发现高浓度的cAMP也具有抑制作用。在再生过程中,这种核苷酸的细胞内浓度会升高,并且在血清素存在的情况下会提前升高。得出的结论是,血清素可能通过一种涉及cAMP和钙离子的机制来调节纤毛再生,但这些化合物之间的因果关系仍有待确定。