Maeda M, Doi O, Akamatsu Y
Biochim Biophys Acta. 1980 Apr 24;597(3):552-63. doi: 10.1016/0005-2736(80)90227-8.
LM cells in which the membrane phospholipids had been modified with choline analogues were infected with vesicular stomatitis virus. The choline analogues tested were choline, N,N'-dimethylethanolamine, N-monomethylethanolamine and ethanolamine. These modifications per se did not affect the syntheses of individual viral proteins. The viral glycoprotein was detected in the plasma membranes of all the modified cells by pronase digestion in pulse-chase experiments, but the amount of glycoprotein susceptible to proteolysis varied, decreasing in these modified cells in the following order: N,N'-dimethylethanolamine- greater than choline- greater than N-monomethylethanolamine- greater than ethanolamine-treated cells. After a 4-h chase, glycoprotein was mainly distributed in the plasma membranes of cells modified with N,N'-dimethylethanolamine, whereas it was found in both the microsomes and plasma membranes of cells modified with other analogues. Fairly large amounts of glycoprotein were also found in the soluble fraction of ethanolamine-treated cells, but not in that of choline- or N,N'-dimethylethanolamine-treated cells. More precise experiments on the behaviour of glycoprotein with a short period of chase strongly suggested that migration of glycoprotein from the microsomes to the plasma membranes was fastest in cells modified with N,N'-dimethylethanolamine and slowest in cells modified with ethanolamine. Membrane lipid modifications also resulted in release of different numbers of progeny virions from the cells, release of virions from the cells decreasing in the following order: N,N'-dimethylethanolamine- greater than choline- greather N-monomethylethanolamine- greater than ethanolamine-treated cells. These results indicate that modification of membrane phospholipids influences not only the insertion of glycoprotein into the microsomes and its migration to the plasma membranes, but also the production of progeny virions.
用胆碱类似物修饰了膜磷脂的LM细胞被水泡性口炎病毒感染。所测试的胆碱类似物有胆碱、N,N'-二甲基乙醇胺、N-单甲基乙醇胺和乙醇胺。这些修饰本身并不影响单个病毒蛋白的合成。在脉冲追踪实验中，通过链霉蛋白酶消化在所有修饰细胞的质膜中检测到病毒糖蛋白，但易受蛋白水解的糖蛋白量有所不同，在这些修饰细胞中，其减少顺序如下：N,N'-二甲基乙醇胺处理的细胞＞胆碱处理的细胞＞N-单甲基乙醇胺处理的细胞＞乙醇胺处理的细胞。经过4小时的追踪后，糖蛋白主要分布在N,N'-二甲基乙醇胺处理的细胞的质膜中，而在用其他类似物修饰的细胞的微粒体和质膜中均能发现。在乙醇胺处理的细胞的可溶性部分中也发现了相当大量的糖蛋白，但在胆碱或N,N'-二甲基乙醇胺处理的细胞中未发现。对糖蛋白短时间追踪行为的更精确实验强烈表明，糖蛋白从微粒体向质膜的迁移在N,N'-二甲基乙醇胺处理的细胞中最快，在乙醇胺处理的细胞中最慢。膜脂质修饰还导致从细胞中释放出不同数量的子代病毒粒子，从细胞中释放病毒粒子的数量减少顺序如下：N,N'-二甲基乙醇胺处理的细胞＞胆碱处理的细胞＞N-单甲基乙醇胺处理的细胞＞乙醇胺处理的细胞。这些结果表明，膜磷脂的修饰不仅影响糖蛋白插入微粒体及其向质膜的迁移，还影响子代病毒粒子的产生。
Zotero 插件