一种用于定量水泡性口炎病毒缺陷干扰颗粒的灵敏方法：蚀斑形成试验。

A sensitive method for quantification of vesicular stomatitis virus defective interfering particles: focus forming assay.

作者信息

Winship T R, Thacore H R

出版信息

J Gen Virol. 1980 May;48(1):237-40. doi: 10.1099/0022-1317-48-1-237.

Abstract

A focus-forming assay for the quantification of defective interfering (DI) particles of vesicular stomatitis virus (VSV) is described. This assay is based on the procedures described for lymphocytic choriomeningitis (LCM) virus by Popescu et al. (1976). Under appropriate conditions this focus-forming assay can quantify fewer than 100 DI particles/ml in a preparation containing a large number of infectious particles.

摘要

本文描述了一种用于定量水泡性口炎病毒（VSV）缺陷干扰（DI）颗粒的蚀斑形成试验。该试验基于Popescu等人（1976年）描述的淋巴细胞性脉络丛脑膜炎（LCM）病毒的程序。在适当条件下，这种蚀斑形成试验可以在含有大量感染性颗粒的制剂中定量少于100个DI颗粒/毫升。