环磷酸腺苷作为儿茶酚胺诱导离体灌注的正常大鼠工作心脏释放酶的介质。

Cyclic AMP as mediator of catecholamine-induced enzyme release from isolated perfused working rat heart.

作者信息

Horak A R, Podzuweit T, Opie L H

出版信息

Adv Myocardiol. 1980;1:367-73.

Abstract

Adrenaline causes a dose-related increase in enzyme (lactate dehydrogenase) release from the isolated perfused working rat heart and a rise in tissue cAMP. Rates of both enzyme release and tissue cAMP fell with beta blockade by propranolol (plus pacing to maintain heart rate). Theophylline also increased enzyme release and tissue cAMP. Dibutyryl cAMP, but not butyric acid or external cAMP, increased enzyme release. Hence the release of enzyme is mediated by cAMP according to the criteria of Sutherland et al (11) for mediating the action of catecholamines. However, factors other than cAMP are operative because the extent of enzyme release and the tissue cAMP can be dissociated by variations in external calcium or magnesium or glucose, which modulate the effects of a given level of cAMP.

摘要

肾上腺素会使离体灌注的正常工作大鼠心脏释放的酶（乳酸脱氢酶）呈剂量相关增加，并使组织中环磷酸腺苷（cAMP）水平升高。普萘洛尔进行β受体阻滞（并通过起搏维持心率）后，酶释放速率和组织cAMP水平均下降。茶碱也会增加酶释放和组织cAMP水平。二丁酰环磷腺苷（dibutyryl cAMP）可增加酶释放，但丁酸或细胞外cAMP则无此作用。因此，根据萨瑟兰等人（11）关于介导儿茶酚胺作用的标准，酶的释放是由cAMP介导的。然而，除cAMP外的其他因素也起作用，因为酶释放程度和组织cAMP水平可因细胞外钙、镁或葡萄糖的变化而解离，这些变化会调节给定cAMP水平的作用。