Leung K L, Yamazaki H
Can J Microbiol. 1980 Jun;26(6):718-21. doi: 10.1139/m80-123.
An Escherichia coli cya mutant deficient in adenylyl cyclase and an E. coli crp mutant deficient in cyclic AMP receptor protein (CRP) accumulate substantial amounts of L-glutamate extracellularly when entering stationary phase of growth. The cya mutant grown in the presence of cyclic AMP accumulates little glutamate whereas the addition of cyclic AMP has no effect on glutamate accumulation in the crp mutant. It is proposed that an E. coli cell entering stationary phase requires a change in cell envelope structure which involes a cyclic AMP-CRP dependent process, and without this process the permeability of the cell membrane increases.
一种缺乏腺苷酸环化酶的大肠杆菌cya突变体和一种缺乏环腺苷酸受体蛋白（CRP）的大肠杆菌crp突变体在进入生长稳定期时会在细胞外积累大量L-谷氨酸。在环腺苷酸存在下生长的cya突变体积累的谷氨酸很少，而添加环腺苷酸对crp突变体中的谷氨酸积累没有影响。有人提出，进入稳定期的大肠杆菌细胞需要改变细胞膜结构，这涉及一个依赖环腺苷酸-CRP的过程，没有这个过程，细胞膜的通透性就会增加。
