Deoxyribonucleases in herpes simplex virus type 1 and 2 infected primary rabbit kidney cells.
作者信息
Zöllner E J, Zahn R K, Falke D
出版信息
Z Naturforsch C Biosci. 1980 Sep-Oct;35(9-10):747-9. doi: 10.1515/znc-1980-9-1016.
PMID:6255696
Abstract
In primary rabbit kidney cells infected with herpes simplex virus four different neutral deoxyribonuclease activities can be detected by means of the deoxyribonuclease assay in DNA-containing polyacrylamide gels following their separation by disc-electrophoresis. The method is suitable to follow independently the change in each activity of the different enzymes using only about 5 X 10(5) cells for each assay during the time-course of infection. Under these conditions one enzyme activity is constant, two disappear while the activity of a fourth one present only in infected cells, increases.
摘要
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