Benedeczky I, Rózsa K S
Histochemistry. 1981;70(2):189-97. doi: 10.1007/BF00493212.
The localization of cyclic 3', 5'-AMP phosphodiesterase was studied by ultrastructural cytochemical methods in the various tissues of Locusta migratoria. Large amounts of electron dense, granular reaction products were detectable on the surface of the corpora allata and the corpus cardiacum, bound to the basal lamina. In the protocerebral neuropile rather large amounts of reaction products were observed in the processes of the glial cells. A significant number of lead phosphate deposit was found to occur on the membrane of certain large axons, the microtubules of the axons, furthermore on the membrane of several terminals. Reaction product was also observable in certain terminals, bound to the synaptic vesicles and the mitochondria. At the same time, electron dense deposits were not detectable at all on the surface of cerebral neurons. In the case of myocardium, reaction product was only found on the basal lamina and the extracellular surface of the sarcolemma. On the basis of our results it can be stated that the cyclic 3', 5'-AMP phosphodiesterase is detectable by cytochemical methods in different tissues of Locusta migratoria and presumably it fulfils the task of the extracellular cAMP level regulation.
采用超微结构细胞化学方法研究了飞蝗不同组织中环3'，5'-AMP磷酸二酯酶的定位。在咽侧体和心侧体表面可检测到大量电子致密的颗粒状反应产物，这些产物附着于基膜上。在原脑神经纤维网中，在神经胶质细胞的突起中观察到相当大量的反应产物。在某些大轴突的膜、轴突的微管以及几个终末的膜上发现有大量磷酸铅沉淀。在某些终末也可观察到反应产物，其附着于突触小泡和线粒体上。与此同时，在脑神经元表面完全检测不到电子致密沉积物。在心肌中，仅在基膜和肌膜的细胞外表面发现反应产物。根据我们的研究结果可以认为，通过细胞化学方法可在飞蝗的不同组织中检测到环3'，5'-AMP磷酸二酯酶，推测它履行细胞外cAMP水平调节的任务。
