环磷酸腺苷受体在人类精子中的差异分布。

Differential distribution of cyclic-AMP receptors in human spermatozoa.

作者信息

Delgado N M, Buacuja L, Merchant H, Rosado A

出版信息

Arch Androl. 1981 Aug;7(1):45-9. doi: 10.3109/01485018109009375.

Abstract

The binding properties of cyclic-AMP to intact and fractionated in heads and tails human spermatozoa have been studied. Whole spermatozoa bound 6.8 +/- 0.8 pmol of cyclic-AMP per 10(7) cells. Binding of cyclic-AMP was selectively located in the midpiece-tail region of the spermatozoa. Thus only 18% of the binding in the whole sperm could be accounted for by the binding on the sperm heads. This distribution is similar to that previously described for the protein kinase of bovine sperm. Blocking membrane sulfhydryl groups with pCMBS induced a drastic inhibition (65%) of the cyclic-AMP binding to the sperm heads, while only moderately reducing (30%) the binding in the sperm tails

摘要

已对环磷酸腺苷（cyclic-AMP）与人精子完整及分离的头部和尾部的结合特性进行了研究。每10⁷个细胞中，完整精子结合6.8±0.8皮摩尔的环磷酸腺苷。环磷酸腺苷的结合选择性地位于精子的中段-尾部区域。因此，整个精子中只有18%的结合可归因于精子头部的结合。这种分布与先前描述的牛精子蛋白激酶的分布相似。用对氯汞苯甲酸（pCMBS）封闭膜巯基会强烈抑制（65%）环磷酸腺苷与精子头部的结合，而仅适度降低（30%）精子尾部的结合。