饱和转移电子顺磁共振中分辨率增强方法的比较。15N同位素取代自旋标记与35GHz高频操作。
A comparison of resolution-enhancement methods in saturation-transfer EPR. 15N isotopically substituted spin labels and 35 GHz high-frequency operation.
作者信息
Johnson M E, Thiyagarajan P, Bates B, Currie B L
出版信息
Biophys J. 1982 Feb;37(2):563-7. doi: 10.1016/s0006-3495(82)84700-0.
Abstract
The use of (15)N isotopic substitution and 35 GHz (Q-band) operation as resolution enhancement methods in saturation-transfer electron paramagnetic resonance (ST-EPR) are compared. We find that both methods offer roughly comparable enhancements in ST-EPR resolution. The most powerful approach to resolving complex ST-EPR spectral behavior, however, will probably be the combined use of multiple-frequency X- and Q-band operation with (15)N isotopically substituted spin labels.
摘要
比较了在饱和转移电子顺磁共振（ST-EPR）中使用（15）N同位素取代和35 GHz（Q波段）操作作为分辨率增强方法的情况。我们发现这两种方法在ST-EPR分辨率方面提供了大致相当的增强效果。然而，解决复杂的ST-EPR光谱行为的最有效方法可能是将多频X波段和Q波段操作与（15）N同位素取代的自旋标记结合使用。
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