Johnson M E, Thiyagarajan P, Bates B, Currie B L
Biophys J. 1982 Feb;37(2):563-7. doi: 10.1016/s0006-3495(82)84700-0.
The use of (15)N isotopic substitution and 35 GHz (Q-band) operation as resolution enhancement methods in saturation-transfer electron paramagnetic resonance (ST-EPR) are compared. We find that both methods offer roughly comparable enhancements in ST-EPR resolution. The most powerful approach to resolving complex ST-EPR spectral behavior, however, will probably be the combined use of multiple-frequency X- and Q-band operation with (15)N isotopically substituted spin labels.
比较了在饱和转移电子顺磁共振(ST-EPR)中使用(15)N同位素取代和35 GHz(Q波段)操作作为分辨率增强方法的情况。我们发现这两种方法在ST-EPR分辨率方面提供了大致相当的增强效果。然而,解决复杂的ST-EPR光谱行为的最有效方法可能是将多频X波段和Q波段操作与(15)N同位素取代的自旋标记结合使用。