与鼠肉瘤病毒转化基因相关的人类核苷酸序列：对克隆的病毒和细胞DNA的研究。

Human nucleotide sequences related to the transforming gene of a murine sarcoma virus: studies with cloned viral and cellular DNAs.

作者信息

Chumakov I M, Zabarovsky E R, Prassolov V S, Mett V L, Kisselev L L

出版信息

Gene. 1982 Jan;17(1):19-26. doi: 10.1016/0378-1119(82)90097-x.

Abstract

A recombinant plasmid, pI26, has been constructed by cloning into pBR322 a transforming gene of murine sarcoma virus (a Moloney strain, clone 124, MSV) synthesized by detergent-treated virions. From this plasmid a XbaI-HindIII fragment has been isolated which contains only mos-specific sequences. This mos-specific probe has been used for screening a human gene library cloned in bacteriophage lambda Charon 4A. Of these, 19 clones have been isolated containing mos-related sequences. By physical mapping and molecular hybridization it has been shown that these sequences are neighboured by DNA regions related to Moloney murine leukemia virus. Recombinant phages have also been found containing human inserts related to MLV, not to the mos gene. The possible existence of murine-like endogenous retroviruses in the normal human genome, including that of a sarcoma type, is discussed. By Northern blotting, expression of the cellular c-mos gene has been detected in mouse liver treated with a hepatocarcinogen. The general significance of the suggested model for evaluating the relationship between chemical carcinogenesis and oncogene expression is discussed.

摘要

通过将经去污剂处理的病毒粒子合成的鼠肉瘤病毒（莫洛尼株，克隆124，MSV）的转化基因克隆到pBR322中，构建了重组质粒pI26。从该质粒中分离出一个XbaI-HindIII片段，其仅包含mos特异性序列。该mos特异性探针已用于筛选克隆在噬菌体λCharon 4A中的人类基因文库。从中分离出了19个含有mos相关序列的克隆。通过物理图谱分析和分子杂交表明，这些序列与莫洛尼鼠白血病病毒相关的DNA区域相邻。还发现了含有与MLV相关而非mos基因的人类插入片段的重组噬菌体。讨论了正常人类基因组中可能存在类似鼠的内源性逆转录病毒，包括肉瘤类型的可能性。通过Northern印迹法，在用肝癌致癌物处理的小鼠肝脏中检测到了细胞c-mos基因的表达。讨论了所提出的模型在评估化学致癌作用与癌基因表达之间关系方面的普遍意义。