Hazum E, Nimrod A
Proc Natl Acad Sci U S A. 1982 Mar;79(6):1747-50. doi: 10.1073/pnas.79.6.1747.
Photoaffinity labeling of rat ovarian granulosa cells and membrane preparations with a bioactive photoaffinity derivative of gonadotropin-releasing hormone resulted in identification of two specific components with apparent molecular weights of 60,000 and 54,000. Fluorescent visualization of gonadotropin-releasing hormone receptors in these cells, by using a bioactive rhodamine derivative of the hormone, indicated that the fluorescently labeled receptors were initially distributed uniformly on the cell surface and then formed patches that subsequently internalized (at 37 degrees C) into endocytic vesicles. These processes were dependent on specific binding sites for the rhodamine-labeled peptide on the granulosa cells. These studies may provide an experimental basis for understanding the molecular events involved in the action of the hormone in the ovary.
用促性腺激素释放激素的一种生物活性光亲和衍生物对大鼠卵巢颗粒细胞和细胞膜制剂进行光亲和标记，结果鉴定出两种表观分子量分别为60,000和54,000的特定成分。通过使用该激素的一种生物活性罗丹明衍生物对这些细胞中的促性腺激素释放激素受体进行荧光可视化显示，荧光标记的受体最初均匀分布在细胞表面，然后形成斑块，随后（在37℃）内化到内吞小泡中。这些过程依赖于颗粒细胞上罗丹明标记肽的特异性结合位点。这些研究可能为理解该激素在卵巢中作用所涉及的分子事件提供实验基础。
